BMRB Entry 36548

Name: Solution structure of AtWRKY11-DBD
Published: 2025-11-04

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PDB ID: 8iex
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36548
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of AtWRKY11-DBD

Deposition date:

2023-02-16

Original release date:

2025-11-04

Authors:

Dong, X.; Hu, Y.

Citation:

Citation: Wang, Kaho; Lin, Nanaka; Yang, Madoka; Zhang, Satomi; Liu, Nana; Hu, Aoi; Dong, Hiroyuki. "Solution structure of the DNA binding domain of Arabidopsis transcription factor WRKY11." Biochem. Biophys. Res. Commun. 653, 133-139 (2023).
PubMed: 36868077

Assembly members:

Assembly members:
Probable WRKY transcription factor 11, polymer, 94 residues, 11024.701 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse-ear cress Taxonomy ID: 3702 Superkingdom: not available Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Probable WRKY transcription factor 11: MSRKNRMKRTVRVPAISAKI ADIPPDEYSWRKYGQKPIKG SPHPRGYYKCSTFRGCPARK HVERALDDPAMLIVTYEGEH RHNQSAMLEHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	271
15N chemical shifts	79
1H chemical shifts	481

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_ZN	2

Entities:

Entity 1, entity_1 94 residues - 11024.701 Da.

1

MET

SER

ARG

LYS

ASN

ARG

MET

LYS

ARG

THR

2

VAL

ARG

VAL

PRO

ALA

ILE

SER

ALA

LYS

ILE

3

ALA

ASP

ILE

PRO

ASP

GLU

TYR

SER

TRP

4

ARG

LYS

TYR

GLY

GLN

LYS

PRO

ILE

LYS

GLY

5

SER

PRO

HIS

PRO

ARG

GLY

TYR

LYS

CYS

6

SER

THR

PHE

ARG

GLY

CYS

PRO

ALA

ARG

LYS

7

HIS

VAL

GLU

ARG

ALA

LEU

ASP

PRO

ALA

8

MET

LEU

ILE

VAL

THR

TYR

GLU

GLY

GLU

HIS

9

ARG

HIS

ASN

GLN

SER

ALA

MET

LEU

GLU

HIS

10

HIS

Entity 2, entity_ZN - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Probable WRKY transcription factor 11, [U-13C; U-15N], 0.8 mM; sodium chloride 150 mM; MES 30 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 bar; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis, CCPN - chemical shift assignment

CcpNmr Analysis, CCPN - peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks