BMRB Entry 36486

Name: Structure of Cyclic Rev
Published: 2025-10-27

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PDB ID: 7xln
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36486
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Cyclic Rev

Deposition date:

2022-04-22

Original release date:

2025-10-27

Authors:

Kim, D.; Lim, Y.; Barnwal, R.

Citation:

Citation: Kim, D.; Lim, Y.; Barnwal, R.. "Structure of Cyclic Rev" .

Assembly members:

Assembly members:
cyclic Rev, polymer, 65 residues, 7642.682 Da.

Natural source:

Natural source: Common Name: HIV-1 Taxonomy ID: 11676 Superkingdom: Viruses Kingdom: Pararnavirae Genus/species: Lentivirus humimdef1

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cyclic Rev: CRGGDEDALKAARAIKFLYQ SNPPPNPEGTRQARRNRRRR WRERQRQIHSASERIASTYL GGMRM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	254
15N chemical shifts	68
1H chemical shifts	299

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 65 residues - 7642.682 Da.

1

CYS

ARG

GLY

ASP

GLU

ASP

ALA

LEU

LYS

2

ALA

ARG

ALA

ILE

LYS

PHE

LEU

TYR

GLN

3

SER

ASN

PRO

ASN

PRO

GLU

GLY

THR

4

ARG

GLN

ALA

ARG

ASN

ARG

5

TRP

ARG

GLU

ARG

GLN

ARG

GLN

ILE

HIS

SER

6

ALA

SER

GLU

ARG

ILE

ALA

SER

THR

TYR

LEU

7

GLY

MET

ARG

MET

Samples:

sample_1: Cyclic Rev, [U-95% 13C; U-95% 15N], 0.15 mM; potassium phosphate 15 mM; potassium chloride 150 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert P. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment

CcpNmr Analysis, CCPN - peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker AVANCE 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks