BMRB Entry 36412

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36412
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title:
SIB1, an effector of Colletotrichum orbiculare
Deposition date:
2021-03-08
Original release date:
2025-10-26
Authors:
Mori, M.; Ohki, S.
Citation:

Citation: Zhang, Ru; Isozumi, Noriyoshi; Mori, Masashi; Okuta, Ryuta; Singkaravanit-Ogawa, Suthitar; Imamura, Tomohiro; Kurita, Jun-Ichi; Gan, Pamela; Shirasu, Ken; Ohki, Shinya; Takano, Yoshitaka. "Fungal effector SIB1 of Colletotrichum orbiculare has unique structural features and can suppress plant immunity in Nicotiana benthamiana."  J. Biol. Chem. 297, 101370-101370 (2021).
PubMed: 34756891

Assembly members:

Assembly members:
SIB1, polymer, 50 residues, 5046.589 Da.

Natural source:

Natural source:   Common Name: Cucumber anthracnose fungus, Colletotrichum lagenarium   Taxonomy ID: 1213857   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Colletotrichum orbiculare

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Nicotiana tabacum

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SIB1: XEGKCTAKGECQENTSGVKL FCTSGSCAKKEGQACTRNGP GSSNSASCPK

Data sets:
Data typeCount
15N chemical shifts47
1H chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 50 residues - 5046.589 Da.

1   PCAGLUGLYLYSCYSTHRALALYSGLYGLU
2   CYSGLNGLUASNTHRSERGLYVALLYSLEU
3   PHECYSTHRSERGLYSERCYSALALYSLYS
4   GLUGLYGLNALACYSTHRARGASNGLYPRO
5   GLYSERSERASNSERALASERCYSPROLYS

Samples:

Unlabeled: SIB1, [U-15N], mM; KCl 100 mM; H2O 90%; D2O, [U-2H], 10%

15N-labeled: SIB1, [U-15N], mM; KCl 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.3 Not defined; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYnot availableisotropicsample_conditions_1
3D 1H-15N TOCSYnot availableisotropicsample_conditions_1
2D 1H-1H NOESYnot availableisotropicsample_conditions_1
2D 1H-1H TOCSYnot availableisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - data analysis

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks