BMRB Entry 36363

Name: Solution structure of NCR169 oxidized form 2 from Medicago truncatula
Published: 2025-10-13

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PDB ID: 7cke
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36363
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of NCR169 oxidized form 2 from Medicago truncatula

Deposition date:

2020-07-16

Original release date:

2025-10-13

Authors:

Isozumi, N.; Masubuchi, Y.; Ohki, S.

Citation:

Citation: Isozumi, Noriyoshi; Masubuchi, Yuya; Imamura, Tomohiro; Mori, Masashi; Koga, Hironori; Ohki, Shinya. "Structure and antimicrobial activity of NCR169, a nodule-specific cysteine-rich peptide of Medicago truncatula." Sci. Rep. 11, 9923-9923 (2021).
PubMed: 33972675

Assembly members:

Assembly members:
Nodule Cysteine-Rich (NCR) secreted peptide, polymer, 39 residues, 4630.519 Da.

Natural source:

Natural source: Common Name: barrel medic Taxonomy ID: 3880 Superkingdom: not available Kingdom: Viridiplantae Genus/species: Medicago truncatula

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nodule Cysteine-Rich (NCR) secreted peptide: GEDIGHIKYCGIVDDCYKSK KPLFKIWKCVENVCVLWYK

Data sets:

assigned_chemical_shifts
- starch_output

Data type	Count
1H chemical shifts	293

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 39 residues - 4630.519 Da.

1

GLY

GLU

ASP

ILE

GLY

HIS

ILE

LYS

TYR

CYS

2

GLY

ILE

VAL

ASP

CYS

TYR

LYS

SER

LYS

3

LYS

PRO

LEU

PHE

LYS

ILE

TRP

LYS

CYS

VAL

4

GLU

ASN

VAL

CYS

VAL

LEU

TRP

TYR

LYS

Samples:

sample_1: peptide 0.6 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: peptide, [U-99% 15N], 0.3 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 mM; pH: 2.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

Bruker AVANCE 800 MHz