BMRB Entry 36312

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36312
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title:
NMR structure and dynamics studies of yeast respiratory super-complex factor 2 in micelles
Deposition date:
2020-01-29
Original release date:
2025-11-01
Authors:
Zhou, S.; Pontus, P.; Peter, B.; Maler, L.; Adelroth, P.
Citation:

Citation: Zhou, Shu; Pettersson, Pontus; Huang, Jingjing; Brzezinski, Peter; Pomes, Regis; Maler, Lena; Adelroth, Pia. "NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2."  Structure 29, 275-283 (2021).
PubMed: 32905793

Assembly members:

Assembly members:
Respiratory supercomplex factor 2, mitochondrial, polymer, 478 residues, 27260.172 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Respiratory supercomplex factor 2, mitochondrial: MKILTQDEIEAHRSHTLKGG IEGALAGFAISAIIFKVLPR RYPKFKPSTLTWSIKTALWI TPPTVLTAICAEEASNNFDA TMYGSGSSSEDALDEHRRWK SLSTKDKFVEGLSNNKYKII TGAWAASLYGSWVIVNKDPI MTKAQKIVQARMYAQFITVG LLLASVGLSMYENKLHPNKQ KVNEMRRWENALRVAEEEER LEKEGRRTGYVSNEERINSK IFKSEFRVPGSHHHHHHHH

Data sets:
Data typeCount
13C chemical shifts778
15N chemical shifts206
1H chemical shifts1012

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1_11
2entity_1_21

Entities:

Entity 1, entity_1_1 478 residues - 27260.172 Da.

1   METLYSILELEUTHRGLNASPGLUILEGLU
2   ALAHISARGSERHISTHRLEULYSGLYGLY
3   ILEGLUGLYALALEUALAGLYPHEALAILE
4   SERALAILEILEPHELYSVALLEUPROARG
5   ARGTYRPROLYSPHELYSPROSERTHRLEU
6   THRTRPSERILELYSTHRALALEUTRPILE
7   THRPROPROTHRVALLEUTHRALAILECYS
8   ALAGLUGLUALASERASNASNPHEASPALA
9   THRMETTYRGLYSERGLYSERSERSERGLU
10   ASPALALEUASPGLUHISARGARGTRPLYS
11   SERLEUSERTHRLYSASPLYSPHEVALGLU
12   GLYLEUSERASNASNLYSTYRLYSILEILE
13   THRGLYALATRPALAALASERLEUTYRGLY
14   SERTRPVALILEVALASNLYSASPPROILE
15   METTHRLYSALAGLNLYSILEVALGLNALA
16   ARGMETTYRALAGLNPHEILETHRVALGLY
17   LEULEULEUALASERVALGLYLEUSERMET
18   TYRGLUASNLYSLEUHISPROASNLYSGLN
19   LYSVALASNGLUMETARGARGTRPGLUASN
20   ALALEUARGVALALAGLUGLUGLUGLUARG
21   LEUGLULYSGLUGLYARGARGTHRGLYTYR
22   VALSERASNGLUGLUARGILEASNSERLYS
23   ILEPHELYSSERGLUPHEARGVALPROGLY
24   SERHISHISHISHISHISHISHISHIS

Samples:

sample_1: Rcf2, [U-99% 15N], 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: Rcf2, 15N_2H, 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: Rcf2, 15N_2H_13C, 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_4: Rcf2, 15N_50%-2H_13C, 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_5: Rcf2, 15N_2H_13C-IVL, 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_6: Rcf2, [U-50% 13C; U-50% 15N; U-14N], 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_7: Rcf2, [U-13C; U-15N], 0.5 mM; NaCl 300 mM; NaOAc 25 mM; Tris 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 25 mM; pH: 5; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
TROSY-HSQCsample_1isotropicsample_conditions_1
Backbone Experimentssample_3isotropicsample_conditions_1
Side-chain Experimentssample_7isotropicsample_conditions_1
Side-chain Experimentssample_4isotropicsample_conditions_1
NOE Experimentssample_4isotropicsample_conditions_1
NOE Experimentssample_7isotropicsample_conditions_1
NOE Experimentssample_6isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

CcpNmr Analysis, CCPN - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks