BMRB Entry 36309

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36309
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of Taf14ET-Sth1EBMC
Deposition date:
2020-01-15
Original release date:
2020-08-31
Authors:
Wu, B.; Chen, G.; Chen, Y.
Citation:

Citation: Chen, Guochao; Wang, Duo; Wu, Bin; Yan, Fuxiang; Xue, Hongjuan; Wang, Quanmeng; Quan, Shu; Chen, Yong. "Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation"  Nat. Commun. 11, 4206-4206 (2020).
PubMed: 32826896

Assembly members:

Assembly members:
Transcription initiation factor TFIID subunit 14, polymer, 72 residues, 8083.089 Da.
Nuclear protein STH1/NPS1, polymer, 60 residues, 6617.640 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Transcription initiation factor TFIID subunit 14: SKGSVDLEKLAFGLTKLNED DLVGVVQMVTDNKTPEMNVT NNVEEGEFIIDLYSLPEGLL KSLWDYVKKNTE
Nuclear protein STH1/NPS1: SEVKSSSVEIINGSESKKKK PKLTVKIKLNKTTVLENNDG KRAEEKPESKSPAKKTAAKY

Data sets:
Data typeCount
13C chemical shifts530
15N chemical shifts121
1H chemical shifts879

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 72 residues - 8083.089 Da.

1   SERLYSGLYSERVALASPLEUGLULYSLEU
2   ALAPHEGLYLEUTHRLYSLEUASNGLUASP
3   ASPLEUVALGLYVALVALGLNMETVALTHR
4   ASPASNLYSTHRPROGLUMETASNVALTHR
5   ASNASNVALGLUGLUGLYGLUPHEILEILE
6   ASPLEUTYRSERLEUPROGLUGLYLEULEU
7   LYSSERLEUTRPASPTYRVALLYSLYSASN
8   THRGLU

Entity 2, entity_2 60 residues - 6617.640 Da.

1   SERGLUVALLYSSERSERSERVALGLUILE
2   ILEASNGLYSERGLUSERLYSLYSLYSLYS
3   PROLYSLEUTHRVALLYSILELYSLEUASN
4   LYSTHRTHRVALLEUGLUASNASNASPGLY
5   LYSARGALAGLUGLULYSPROGLUSERLYS
6   SERPROALALYSLYSTHRALAALALYSTYR

Samples:

sample_1: Phosphate buffer 20 mM; Sth1EBMC, [U-100% 13C; U-100% 15N], 1.5 mM; Taf14ET, [U-100% 13C; U-100% 15N], 1 mM; inhabitor cocktail 1 % v/v; sodium azide 0.04%; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

Sparky, Goddard - data analysis

NMR spectrometers:

  • Agilent DD2 800 MHz
  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks