BMRB Entry 36275

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36275
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title:
Structure of single disulfide peptide Czon1107-WT (major conformer)
Deposition date:
2019-08-02
Original release date:
2025-10-22
Authors:
Sarma, S.; Madhan Kumar, M.
Citation:

Citation: Mohan, Madhan Kumar; Abraham, Nikita; R, Rajesh; Jayaseelan, Benajamin Franklin; Ragnarsson, Lotten; Lewis, Richard; Sarma, Siddhartha. "Structure and allosteric activity of a single-disulfide conopeptide from Conus zonatus at human a3b4 and a7 nicotinic acetylcholine receptors."  J. Biol. Chem. 295, 7096-7112 (2020).
PubMed: 32234761

Assembly members:

Assembly members:
Czon1107-WT (Conformer A), polymer, 11 residues, 1109.324 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 754466   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus zonatus

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: unidentified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Czon1107-WT (Conformer A): GFRSPCPPFCX

Data sets:
Data typeCount
13C chemical shifts31
15N chemical shifts7
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 11 residues - 1109.324 Da.

1   GLYPHEARGSERPROCYSPROPROPHECYS
2   NH2

Samples:

sample_1: Czon1107-WT major conformer 3.0 ± 0.3 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 Not defined; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCTOCSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

CcpNmr Analysis, CCPN - peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks