BMRB Entry 36179

Name: Solution structure of centrin4 from Trypanosoma brucei
Published: 2019-03-11

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PDB ID: 5zor
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36179
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of centrin4 from Trypanosoma brucei

Deposition date:

2018-04-15

Original release date:

2019-03-11

Authors:

Shan, F.; Tu, X.

Citation:

Citation: Shan, Fangzhen; Ye, Kaiqin; Zhang, Jiahai; Liao, Shanhui; Zhang, Xuecheng; Xu, Chao; Tu, Xiaoming. "Solution structure of TbCentrin4 from" Biochem. J. 475, 3763-3778 (2018).
PubMed: 30389845

Assembly members:

Assembly members:
Centrin, putative, polymer, 149 residues, 16570.627 Da.

Natural source:

Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 185431 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma Trypanosoma brucei

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Centrin, putative: MAALTDEQIREAFNLFDADG SGAIDAEEMALAMKGLGFGD LPRDEVERTVRSMNTNANGL IEYGEFERMVKSRMAQKDSP EEVLKAFQLFDLDKKGKISF ANLKEVAKLLGENPGDDVLQ EMIAEADEDGDGEVSFEEFK SVMMQMRGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	368
15N chemical shifts	145
1H chemical shifts	960

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 149 residues - 16570.627 Da.

1

MET

ALA

LEU

THR

ASP

GLU

GLN

ILE

ARG

2

GLU

ALA

PHE

ASN

LEU

PHE

ASP

ALA

ASP

GLY

3

SER

GLY

ALA

ILE

ASP

ALA

GLU

MET

ALA

4

LEU

ALA

MET

LYS

GLY

LEU

GLY

PHE

GLY

ASP

5

LEU

PRO

ARG

ASP

GLU

VAL

GLU

ARG

THR

VAL

6

ARG

SER

MET

ASN

THR

ASN

ALA

ASN

GLY

LEU

7

ILE

GLU

TYR

GLY

GLU

PHE

GLU

ARG

MET

VAL

8

LYS

SER

ARG

MET

ALA

GLN

LYS

ASP

SER

PRO

9

GLU

VAL

LEU

LYS

ALA

PHE

GLN

LEU

PHE

10

ASP

LEU

ASP

LYS

GLY

LYS

ILE

SER

PHE

11

ALA

ASN

LEU

LYS

GLU

VAL

ALA

LYS

LEU

12

GLY

GLU

ASN

PRO

GLY

ASP

VAL

LEU

GLN

13

GLU

MET

ILE

ALA

GLU

ALA

ASP

GLU

ASP

GLY

14

ASP

GLY

GLU

VAL

SER

PHE

GLU

PHE

LYS

15

SER

VAL

MET

GLN

MET

ARG

GLY

LYS

Samples:

sample_1: centrin4, [U-13C; U-15N], 0.5 mM; sodium chloride 200 mM; sodium phosphate 20 mM; DTT 2 mM; EDTA 2 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert P., Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks