BMRB Entry 36117

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36117
MolProbity Validation Chart

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Title:
SOLUTION STRUCTURE OF HUMAN MOG1
Deposition date:
2017-09-21
Original release date:
2018-02-06
Authors:
Hu, Q.; Liu, Y.; Bao, X.; Liu, H.
Citation:

Citation: Bao, X.; Liu, H.; Liu, X.; Ruan, K.; Zhang, Y.; Zhang, Z.; Hu, Q.; Liu, Y.; Akram, S.; Zhang, J.; Gong, Q.; Wang, W.; Yuan, X.; Dou, Z.; Tian, R.; Yao, X.; Wu, J.; Shi, Y.. "Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation"  J. Mol. Cell. Biol. 10, 18-32 (2018).
PubMed: 29040603

Assembly members:

Assembly members:
Ran guanine nucleotide release factor, polymer, 194 residues, 21523.146 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: PET22B+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ran guanine nucleotide release factor: MEPTRDCPLFGGAFSAILPM GAIDVSDLRPVPDNQEVFCH PVTDQSLIVELLELQAHVRG EAAARYHFEDVGGVQGARAV HVESVQPLSLENLALRGRCQ EAWVLSGKQQIAKENQQVAK DVTLHQALLRLPQYQTDLLL TFNQPPPDNRSSLGPENLSP APWSLGDFEQLVTSLTLHDP NIFGPQVEHHHHHH

Data sets:
Data typeCount
13C chemical shifts569
15N chemical shifts160
1H chemical shifts909

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 194 residues - 21523.146 Da.

1   METGLUPROTHRARGASPCYSPROLEUPHE
2   GLYGLYALAPHESERALAILELEUPROMET
3   GLYALAILEASPVALSERASPLEUARGPRO
4   VALPROASPASNGLNGLUVALPHECYSHIS
5   PROVALTHRASPGLNSERLEUILEVALGLU
6   LEULEUGLULEUGLNALAHISVALARGGLY
7   GLUALAALAALAARGTYRHISPHEGLUASP
8   VALGLYGLYVALGLNGLYALAARGALAVAL
9   HISVALGLUSERVALGLNPROLEUSERLEU
10   GLUASNLEUALALEUARGGLYARGCYSGLN
11   GLUALATRPVALLEUSERGLYLYSGLNGLN
12   ILEALALYSGLUASNGLNGLNVALALALYS
13   ASPVALTHRLEUHISGLNALALEULEUARG
14   LEUPROGLNTYRGLNTHRASPLEULEULEU
15   THRPHEASNGLNPROPROPROASPASNARG
16   SERSERLEUGLYPROGLUASNLEUSERPRO
17   ALAPROTRPSERLEUGLYASPPHEGLUGLN
18   LEUVALTHRSERLEUTHRLEUHISASPPRO
19   ASNILEPHEGLYPROGLNVALGLUHISHIS
20   HISHISHISHIS

Samples:

sample_2: Mog1, [U-13C], 0.9 ± 0.1 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: Mog1, [U-99% 13C; U-99% 15N], 0.9 ± 0.1 mM; D2O, [U-2H], 100%

sample_1: Mog1, [U-99% 15N], 0.9 ± 0.1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D CBCANHsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-COSYsample_3isotropicsample_conditions_1
3D C(CO)NH-TOCSYsample_3isotropicsample_conditions_1
3D HC(CO)NH-TOCSYsample_3isotropicsample_conditions_1
3D HBHA(CO)NHsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1

Software:

CNS, BRUNGER, ADAMS, CLORE, GROS, NILGES, READ - refinement

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

Procheck, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Thornton - data analysis

SPARKY, Goddard - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks