BMRB Entry 36102

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36102
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
Solution Structure of Cold Shock Protein from Colwellia psychrerythraea
Deposition date:
2017-06-27
Original release date:
2025-10-10
Authors:
Lee, Y.; Kim, Y.
Citation:

Citation: Lee, Yeongjoon; Kwak, Chulhee; Jeong, Ki-Woong; Durai, Prasannavenkatesh; Ryu, Kyoung-Seok; Kim, Eun-Hee; Cheong, Chaejoon; Ahn, Hee-Chul; Kim, Hak; Kim, Yangmee. "Tyr51: Key Determinant of the Low Thermostability of the Colwellia psychrerythraea Cold-Shock Protein."  Biochemistry 57, 3625-3640 (2018).
PubMed: 29737840

Assembly members:

Assembly members:
Cold-shock DNA-binding domain family protein, polymer, 68 residues, 7299.082 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 167879   Superkingdom: Bacteria   Kingdom: Pseudomonadati   Genus/species: Colwellia psychrerythraea

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cold-shock DNA-binding domain family protein: MSKGIVKWFNSDKGFGFITP EDGSKDLFVHHSEIQSGGEY ATLADGQTVEYEVGQGQKGP CANKVVAV

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts71
1H chemical shifts415

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 68 residues - 7299.082 Da.

1   METSERLYSGLYILEVALLYSTRPPHEASN
2   SERASPLYSGLYPHEGLYPHEILETHRPRO
3   GLUASPGLYSERLYSASPLEUPHEVALHIS
4   HISSERGLUILEGLNSERGLYGLYGLUTYR
5   ALATHRLEUALAASPGLYGLNTHRVALGLU
6   TYRGLUVALGLYGLNGLYGLNLYSGLYPRO
7   CYSALAASNLYSVALVALALAVAL

Samples:

sample_1: Colwellia psychrerythraea Cold Shock Protein, [U-99% 15N], 0.5 ± 0.2 mM; sodium azide 0.5 mg/mL; DTT 2 mM; EDTA 0.1 mM; potassium chloride 100 mM; potassium phosphate 50 mM; DSS 50 nM; H2O 90%; D2O, [U-2H], 10%

sample_2: Colwellia psychrerythraea Cold Shock Protein, [U-99% 13C; U-99% 15N], 0.5 ± 0.2 mM; sodium azide 0.5 mg/mL; DTT 2 mM; EDTA 0.1 mM; potassium chloride 100 mM; potassium phosphate 50 mM; DSS 50 nM; H2O 90%; D2O, [U-2H], 10%

sample_3: Colwellia psychrerythraea Cold Shock Protein, [U-99% 15N], 0.5 ± 0.2 mM; sodium azide 0.5 mg/mL; DTT 2 mM; EDTA 0.1 mM; potassium chloride 100 mM; potassium phosphate 50 mM; DSS 50 nM; H2O 90%; D2O, [U-2H], 10%

sample_4: Colwellia psychrerythraea Cold Shock Protein 0.5 ± 0.2 mM; sodium azide 0.5 mg/mL; DTT 2 mM; EDTA 0.1 mM; potassium chloride 100 mM; potassium phosphate 50 mM; DSS 50 nM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D 1H-15N HSQC-DSSE(IPAP)sample_1isotropicsample_conditions_1
2D 1H-15N HSQC-DSSE(IPAP)sample_3anisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRFAM-SPARKY, Lee, W., M. Tonelli and J.L. Markley - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

PONDEROSA-C/S, Lee W, Cornilescu G, Dashti H, Eghbalnia HR, Tonelli M, Westler WM, Butcher SE, Wildman-Henzler K, Markley JL. - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks