BMRB Entry 36085

Name: Anti-CRISPR protein AcrIIA4
Published: 2018-07-17

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PDB ID: 5xn4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36085
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Anti-CRISPR protein AcrIIA4

Deposition date:

2017-05-17

Original release date:

2018-07-17

Authors:

Suh, J.-Y., .; Kim, I.

Citation:

Citation: Kim, I.; Jeong, M.; Ka, D.; Han, M.; Kim, N.; Bae, E.; Suh, J.. "Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor." Sci. Rep. 8, 3883-3883 (2018).
PubMed: 29497118

Assembly members:

Assembly members:
Anti-CRISPR AcrIIA4, polymer, 87 residues, 10182.073 Da.

Natural source:

Natural source: Common Name: Listeria monocytogenes Taxonomy ID: 1639 Superkingdom: Bacteria Kingdom: not available Genus/species: Listeria monocytogenes

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-CRISPR AcrIIA4: MNINDLIREIKNKDYTVKLS GTDSNSITQLIIRVNNDGNE YVISESENESIVEKFISAFK NGWNQEYEDEEEFYNDMQTI TLKSELN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	97
1H chemical shifts	589

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 87 residues - 10182.073 Da.

1

MET

ASN

ILE

ASN

ASP

LEU

ILE

ARG

GLU

ILE

2

LYS

ASN

LYS

ASP

TYR

THR

VAL

LYS

LEU

SER

3

GLY

THR

ASP

SER

ASN

SER

ILE

THR

GLN

LEU

4

ILE

ARG

VAL

ASN

ASP

GLY

ASN

GLU

5

TYR

VAL

ILE

SER

GLU

SER

GLU

ASN

GLU

SER

6

ILE

VAL

GLU

LYS

PHE

ILE

SER

ALA

PHE

LYS

7

ASN

GLY

TRP

ASN

GLN

GLU

TYR

GLU

ASP

GLU

8

GLU

PHE

TYR

ASN

ASP

MET

GLN

THR

ILE

9

THR

LEU

LYS

SER

GLU

LEU

ASN

Samples:

sample_1: AcrIIA4, [U-99% 13C; U-99% 15N], 2.0 mM; Tris HCl 20 mM; NaCl 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

PIPP, Garrett - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks