BMRB Entry 36062

Name: N-terminal Zinc Finger of Synaptotagmin-like Protein 4
Published: 2017-10-27

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PDB ID: 5x6t
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36062
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-terminal Zinc Finger of Synaptotagmin-like Protein 4

Deposition date:

2017-02-23

Original release date:

2017-10-27

Authors:

Miyamoto, K.

Citation:

Citation: Miyamoto, K.; Nakatani, A.; Saito, K.. "The unique N-terminal zinc finger of Synaptotagmin-like protein 4 reveals FYVE structure" Protein Sci. 26, 2451-2457 (2017).
PubMed: 28906046

Assembly members:

Assembly members:
Synaptotagmin-like protein 4, polymer, 53 residues, 6040.966 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Synaptotagmin-like protein 4: SDRTCARCQESLGRLSPKTN TCRGCNHLVCRDCRIQESNG TWRCKVCAKEIEL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_ZN_1	2
3	entity_ZN_2	2

Entities:

Entity 1, entity_1 53 residues - 6040.966 Da.

1

SER

ASP

ARG

THR

CYS

ALA

ARG

CYS

GLN

GLU

2

SER

LEU

GLY

ARG

LEU

SER

PRO

LYS

THR

ASN

3

THR

CYS

ARG

GLY

CYS

ASN

HIS

LEU

VAL

CYS

4

ARG

ASP

CYS

ARG

ILE

GLN

GLU

SER

ASN

GLY

5

THR

TRP

ARG

CYS

LYS

VAL

CYS

ALA

LYS

GLU

6

ILE

GLU

LEU

Entity 2, entity_ZN_1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Synaptotagmin-like Protein 4 1 mM; 1,4-DL-dithiothreitol, [U-2H], 1 mM; NaCl 50 mM; Tris-HCl, [U-2H], 20 mM; ZnCl2 50 uM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 250 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRVIEW - structure solution

NMR spectrometers:

Bruker Avance 500 MHz