BMRB Entry 36026

Name: Solution structure of human Gelsolin protein domain 1 at pH 5.0
Published: 2017-10-30

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PDB ID: 5h3m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36026
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of human Gelsolin protein domain 1 at pH 5.0

Deposition date:

2016-10-26

Original release date:

2017-10-30

Authors:

Fan, J.; Yang, D.

Citation:

Citation: Fan, Jing-Song; Goh, Honzhen; Ding, Ke; Xue, Bo; Robinson, Robert; Yang, Daiwen. "Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1." Sci. Rep. 7, 45230-45230 (2017).
PubMed: 28349924

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, 14973.815 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EHPEFLKAGKEPGLQIWRVE KFDLVPVPTNLYGDFFTGDA YVILKTVQLRNGNLQYDLHY WLGNECSQDESGAAAIFTVQ LDDYLNGRAVQHREVQGFES ATFLGYFKSGLKYKKGGVAS GFKHVVPNEVVVQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	114
15N chemical shifts	127
1H chemical shifts	742

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 133 residues - 14973.815 Da.

1

GLU

HIS

PRO

GLU

PHE

LEU

LYS

ALA

GLY

LYS

2

GLU

PRO

GLY

LEU

GLN

ILE

TRP

ARG

VAL

GLU

3

LYS

PHE

ASP

LEU

VAL

PRO

VAL

PRO

THR

ASN

4

LEU

TYR

GLY

ASP

PHE

THR

GLY

ASP

ALA

5

TYR

VAL

ILE

LEU

LYS

THR

VAL

GLN

LEU

ARG

6

ASN

GLY

ASN

LEU

GLN

TYR

ASP

LEU

HIS

TYR

7

TRP

LEU

GLY

ASN

GLU

CYS

SER

GLN

ASP

GLU

8

SER

GLY

ALA

ILE

PHE

THR

VAL

GLN

9

LEU

ASP

TYR

LEU

ASN

GLY

ARG

ALA

VAL

10

GLN

HIS

ARG

GLU

VAL

GLN

GLY

PHE

GLU

SER

11

ALA

THR

PHE

LEU

GLY

TYR

PHE

LYS

SER

GLY

12

LEU

LYS

TYR

LYS

GLY

VAL

ALA

SER

13

GLY

PHE

LYS

HIS

VAL

PRO

ASN

GLU

VAL

14

VAL

GLN

Samples:

sample_1: gelsolin domain 1, [U-13C; U-15N], 0.65 ± 0.1 mM; Acetate 10 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
4D 13C_15N_edited NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.13, Goddard - data analysis, peak picking

XPLOR-NIH, C.D. Schwieters, J.J. Kuszewski - structure calculation

NMRPipe v8.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks