BMRB Entry 35075
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR35075
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Trappetti, C.; van Ingen, H.; Falconi, M.; Romeo, A.; Sancamillo, G.; Caprini, M.; Oggioni, M.; Sette, M.. "Structured peptides as vaccine antigens against infections
" Front. Immunol. 17, .-. (2026).
Assembly members:
entity_1, polymer, 26 residues, 3164.460 Da.
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: SWTWENGKWTWKCTSDQDEQ
FIPKGC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 66 |
| 1H chemical shifts | 153 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 26 residues - 3164.460 Da.
| 1 | SER | TRP | THR | TRP | GLU | ASN | GLY | LYS | TRP | THR | ||||
| 2 | TRP | LYS | CYS | THR | SER | ASP | GLN | ASP | GLU | GLN | ||||
| 3 | PHE | ILE | PRO | LYS | GLY | CYS |
Samples:
sample_1: trpzip2PAK 0.69 mM
sample_conditions_1: ionic strength: 28.68 mM; pH: 6.1; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRFAM-SPARKY, Goddard-Markley - chemical shift assignment, peak picking
CYANA, Guntert - refinement, structure calculation
NMR spectrometers:
- Bruker AVANCE NEO 900 MHz