BMRB Entry 35009
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR35009
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Falcigno, L.; D'Auria, G.; Galdiero, S.; Falanga, A.; Bellavita, R.. "NMR structures in solution of Temporizin-1" .
Assembly members:
entity_1, polymer, 13 residues, 1802.275 Da.
Natural source: Common Name: Rana temporaria Taxonomy ID: 8407 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rana temporaria
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: FLPLWLWLWRKLK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 13 residues - 1802.275 Da.
| 1 | PHE | LEU | PRO | LEU | TRP | LEU | TRP | LEU | TRP | ARG | ||||
| 2 | LYS | LEU | LYS |
Samples:
sample_1: Temporizin-1 0.55 mM
sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
NMR spectrometers:
- Bruker AVANCE 600 MHz