BMRB Entry 34918

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34918
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
SOLUTION STRUCTURE OF 6XHIS-TAGGED WILD-TYPE GAUSSIA LUCIFERASE
Deposition date:
2024-06-04
Original release date:
2024-07-12
Authors:
Dijkema, F.; Prestel, A.; Winther, J.; Teilum, K.
Citation:

Citation: Dijkema, F.; Escarpizo-Lorenzanam, M.; Nordentoft, M.; Rabe, H.; Sahin, C.; Landreh, M.; Branca, R.; Sorensen, E.; Christensen, B.; Prestel, A.; Teilum, K.; Winther, J.. "A suicidal and extensively disordered luciferase with a bright luminescence"  .

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, 19155.137 Da.

Natural source:

Natural source:   Common Name: Gaussia princeps   Taxonomy ID: 148582   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gaussia princeps

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHKPTENNEDFNIVA VASNFATTDLDADRGKLPGK KLPLEVLKEMEANARKAGCT RGCLICLSHIKCTPKMKKFI PGRCHTYEGDKESAQGGIGE AIVDIPEIPGFKDLEPMEQF IAQVDLCVDCTTGCLKGLAN VQCSDLLKKWLPQRCATFAS KIQGQVDKIKGAGGD

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts129
1H chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 175 residues - 19155.137 Da.

1   METHISHISHISHISHISHISLYSPROTHR
2   GLUASNASNGLUASPPHEASNILEVALALA
3   VALALASERASNPHEALATHRTHRASPLEU
4   ASPALAASPARGGLYLYSLEUPROGLYLYS
5   LYSLEUPROLEUGLUVALLEULYSGLUMET
6   GLUALAASNALAARGLYSALAGLYCYSTHR
7   ARGGLYCYSLEUILECYSLEUSERHISILE
8   LYSCYSTHRPROLYSMETLYSLYSPHEILE
9   PROGLYARGCYSHISTHRTYRGLUGLYASP
10   LYSGLUSERALAGLNGLYGLYILEGLYGLU
11   ALAILEVALASPILEPROGLUILEPROGLY
12   PHELYSASPLEUGLUPROMETGLUGLNPHE
13   ILEALAGLNVALASPLEUCYSVALASPCYS
14   THRTHRGLYCYSLEULYSGLYLEUALAASN
15   VALGLNCYSSERASPLEULEULYSLYSTRP
16   LEUPROGLNARGCYSALATHRPHEALASER
17   LYSILEGLNGLYGLNVALASPLYSILELYS
18   GLYALAGLYGLYASP

Samples:

sample_1: GAUSSIA LUCIFERASE, [U-15N; U-13C], 0.8 mM; sodium azide 0.02%; sodium dihydrogen phosphate 50 mM; sodium trimethylsilylpropanesulfonate (DSS) 0.125 mM; sodium hydroxide 7.5 mM

sample_conditions_1: ionic strength: 57.6 mM; pH: 6.0; pressure: 1 atm; temperature: 308.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNmr Analysis, CcpNmr - peak picking

NMR spectrometers:

  • Bruker AVANCE III HD 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks