BMRB Entry 34910

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34910
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR solution structure of lipid transfer protei Sola l7 from tomato seeds
Deposition date:
2024-03-18
Original release date:
2024-08-28
Authors:
Parron-Ballesteros, J.; Mantin-Pedraz, L.; G.Gordo, R.; Mayorga, C.; Villaba, M.; Batanero, E.; Pantoja-Uceda, D.; Turnay, J.
Citation:

Citation: Parron-Ballesteros, J.; Mantin-Pedraz, L.; G.Gordo, R.; Mayorga, C.; Villalba, M.; Batanero, E.; Pantoja-Uceda, D.; Turnay, J.. "NMR solution structure of lipid transfer protei Sola l7 from tomato seeds"  .

Assembly members:

Assembly members:
entity_1, polymer, 92 residues, 9453.610 Da.

Natural source:

Natural source:   Common Name: tomato   Taxonomy ID: 4081   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Solanum lycopersicum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Komagataella pastoris

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AITCGQVDANLAPCVPFLTQ GGEPGAACCSGVRTLNGNTQ SSDDRRTACNCVKAAANRYP NLKDDAAQSLPSKCGISLTV PISRTVNCDTIS

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts95
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 92 residues - 9453.610 Da.

1   ALAILETHRCYSGLYGLNVALASPALAASN
2   LEUALAPROCYSVALPROPHELEUTHRGLN
3   GLYGLYGLUPROGLYALAALACYSCYSSER
4   GLYVALARGTHRLEUASNGLYASNTHRGLN
5   SERSERASPASPARGARGTHRALACYSASN
6   CYSVALLYSALAALAALAASNARGTYRPRO
7   ASNLEULYSASPASPALAALAGLNSERLEU
8   PROSERLYSCYSGLYILESERLEUTHRVAL
9   PROILESERARGTHRVALASNCYSASPTHR
10   ILESER

Samples:

sample_1: rSolal7, [U-100% 13C; U-100% 15N], 0.7 mM; DSS 1%

sample_2: rSolal7, [U-100% 13C; U-100% 15N], 0.7 mM; DSS 1%

sample_3: rSolal7, [U-100% 13C; U-100% 15N], 0.7 mM; DSS 1%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOisample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAisample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HC(C)-TOCSYsample_1isotropicsample_conditions_1
3D H(NCOCA)NHsample_1isotropicsample_conditions_1
3D (H)N(COCA)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

TALOS+, Yang Shen, Frank Delaglio, Gabriel Cornilescu & Ad Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

PyMOL, Schrodinger - data analysis

MOLMOL, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks