BMRB Entry 34874

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PDB ID: 8r1x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34874
MolProbity Validation Chart

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Title:
Solution structure and chemical shift assignments for HMG-D Y12F mutant complexed to a 14:12 dA2 bulge DNA
Deposition date:
2023-11-02
Original release date:
2024-09-05
Authors:
Yang, J.; Hill, G.; Neuhaus, D.
Citation:

Citation: Hill, Guy; Yang, Ji-Chun; Easton, Laura; Cerdan, Rachel; McLaughlin, Stephen; Stott, Katherine; Travers, Andrew; Neuhaus, David. "A Single Interfacial Point Mutation Rescues Solution Structure Determination of the Complex of HMG-D with a DNA Bulge"  Chembiochem 25, e202400395-e202400395 (2024).
PubMed: 39145407

Assembly members:

Assembly members:
entity_1, polymer, 112 residues, 12426.941 Da.
entity_2, polymer, 14 residues, 4288.817 Da.
entity_3, polymer, 12 residues, 3662.404 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MSDKPKRPLSAFMLWLNSAR ESIKRENPGIKVTEVAKRGG ELWRAMKDKSEWEAKAAKAK DDYDRAVKEFEANGGSSAAN GGGAKKRAKPAKKVAKKSKK EESDEDDDDESE
entity_2: CGATATTAAGAGCC
entity_3: GGCTCAATATCG

Data sets:
Data typeCount
13C chemical shifts259
15N chemical shifts90
1H chemical shifts750

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_33

Entities:

Entity 1, unit_1 112 residues - 12426.941 Da.

1   METSERASPLYSPROLYSARGPROLEUSER
2   ALAPHEMETLEUTRPLEUASNSERALAARG
3   GLUSERILELYSARGGLUASNPROGLYILE
4   LYSVALTHRGLUVALALALYSARGGLYGLY
5   GLULEUTRPARGALAMETLYSASPLYSSER
6   GLUTRPGLUALALYSALAALALYSALALYS
7   ASPASPTYRASPARGALAVALLYSGLUPHE
8   GLUALAASNGLYGLYSERSERALAALAASN
9   GLYGLYGLYALALYSLYSARGALALYSPRO
10   ALALYSLYSVALALALYSLYSSERLYSLYS
11   GLUGLUSERASPGLUASPASPASPASPGLU
12   SERGLU

Entity 2, unit_2 14 residues - 4288.817 Da.

1   DCDGDADTDADTDTDADADG
2   DADGDCDC

Entity 3, unit_3 12 residues - 3662.404 Da.

1   DGDGDCDTDCDADADTDADT
2   DCDG

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