BMRB Entry 34838

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34838
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Short RNA binding to peptide amyloids
Deposition date:
2023-07-24
Original release date:
2023-11-06
Authors:
Rout, S.; Cadalbert, R.; Schroder, N.; Wiegand, T.; Zehnder, J.; Gampp, O.; Guntert, P.; Kringler, D.; Kreutz, C.; Knorlein, A.; Hall, J.; Greenwald, J.; Riek, R.
Citation:

Citation: Rout, S.; Cadalbert, R.; Schroder, N.; Wang, J.; Zehnder, J.; Gampp, O.; Wiegand, T.; Guntert, P.; Klingler, D.; Kreutz, C.; Knorlein, A.; Hall, J.; Greenwald, J.; Riek, R.. "An Analysis of Nucleotide-Amyloid Interactions Reveals Selective Binding to Codon-Sized RNA."  J. Am. Chem. Soc. 145, 21915-21924 (2023).
PubMed: 37782045

Assembly members:

Assembly members:
entity_1, polymer, 9 residues, 853.985 Da.
entity_2, polymer, 4 residues, 1240.802 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: VAQAQINIX
entity_2: GUCA

Data sets:
Data typeCount
13C chemical shifts39

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_21
3unit_31
4unit_41
5unit_51
6unit_61
7unit_71
8unit_81
9unit_91
10unit_101
11unit_112

Entities:

Entity 1, unit_1 9 residues - 853.985 Da.

1   VALALAGLNALAGLNILEASNILENH2

Entity 2, unit_11 4 residues - 1240.802 Da.

1   GUCA

Samples:

sample_1: VAL-ALA-GLN-ALA-GLN-ILE-ASN-ILE, [U-13C; U-15N], 10 na; RNA (5'-R(P*GP*UP*CP*A)-3'), [U-13C; U-15N], 1.5 na

sample_2: VAL-ALA-GLN-ALA-GLN-ILE-ASN-ILE, mixed, 10 na; RNA (5'-R(P*GP*UP*CP*A)-3'), [U-13C; U-15N], 1.5 na

sample_3: VAL-ALA-GLN-ALA-GLN-ILE-ASN-ILE, mixed, 10 na; RNA (5'-R(P*GP*UP*CP*A)-3'), [U-13C; U-15N], 1.5 na

sample_4: VAL-ALA-GLN-ALA-GLN-ILE-ASN-ILE, [U-13C; U-15N], 10 na; RNA (5'-R(P*GP*UP*CP*A)-3') 1.5 na

sample_5: VAL-ALA-GLN-ALA-GLN-ILE-ASN-ILE, [U-13C; U-15N], 10 na; RNA (5'-R(P*GP*UP*CP*A)-3'), specificly labeled, 1.5 na

sample_conditions_1: ionic strength: 0.1 Not defined; pH: 3; pressure: 1 atm; temperature: 272 K

Experiments:

NameSampleSample stateSample conditions
DARR 20 mssample_1anisotropicsample_conditions_1
DARR 150 mssample_1anisotropicsample_conditions_1
NCAsample_1anisotropicsample_conditions_1
NCOsample_1anisotropicsample_conditions_1
DARR 20 mssample_2anisotropicsample_conditions_1
CHHPsample_4anisotropicsample_conditions_1
PAIN 6 mssample_3anisotropicsample_conditions_1
CHHCsample_2anisotropicsample_conditions_1
CHHCsample_1anisotropicsample_conditions_1
DARR 450 mssample_5anisotropicsample_conditions_1
DARR 150 mssample_5anisotropicsample_conditions_1
NCOsample_4anisotropicsample_conditions_1
NCAsample_4anisotropicsample_conditions_1
DARR 20 mssample_4anisotropicsample_conditions_1
DARR 20 mssample_3anisotropicsample_conditions_1
PDSDsample_3anisotropicsample_conditions_1
PAR 8 mssample_3anisotropicsample_conditions_1
DARR 150 mssample_2anisotropicsample_conditions_1
31P DPsample_4anisotropicsample_conditions_1
31P PDSDsample_4anisotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment, peak picking

CYANA v3.98.15, P. Guntert - structure calculation

NMR spectrometers:

  • Bruker AVANCE III 850 MHz