BMRB Entry 34755

Title:
NMR structure of the antimicrobial peptide Of-Pis1 in DPC micelles
Deposition date:
2022-09-10
Original release date:
2022-11-28
Authors:
Alaimo, N.; Bischetti, M.; Gallo, M.; Cicero, D.
Citation:

Citation: Bischetti, M.; Alaimo, N.; Nardelli, F.; Punzi, P.; Amiariei, C.; Ingenito, R.; Musco, G.; Gallo, M.; Cicero, D.. "Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes"  Biochim. Biophys. Acta Biomembr. 1865, 184080-184080 (2023).
PubMed: 36328080

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, 2583.042 Da.

Natural source:

Natural source:   Common Name: barred knifejaw   Taxonomy ID: 163134   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oplegnathus fasciatus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: FLGMLLHGVGHAIHGLIHGK QNVE

Data sets:
Data typeCount
13C chemical shifts84
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 24 residues - 2583.042 Da.

1   PHELEUGLYMETLEULEUHISGLYVALGLY
2   HISALAILEHISGLYLEUILEHISGLYLYS
3   GLNASNVALGLU

Samples:

sample_1: Of-Pis1 3 mM; DPC, [U-2H], 60 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 40 mM; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

NMR spectrometers:

  • Bruker AVANCE 600 MHz