BMRB Entry 34703

Title:
Solution structure of Tk-hefu-11
Deposition date:
2022-01-26
Original release date:
2022-09-21
Authors:
Mineev, K.; Lushpa, V.; Vassilevski, A.; Gigolaev, A.
Citation:

Citation: Gigolaev, Andrei; Lushpa, Vladislav; Pinheiro-Junior, Ernesto; Tabakmakher, Valentin; Peigneur, Steve; Ignatova, Anastasija; Feofanov, Alexey; Efremov, Roman; Mineev, Konstantin; Tytgat, Jan; Vassilevski, Alexander. "Artificial pore blocker acts specifically on voltage-gated potassium channel isoform K V 1.6"  J. Biol. Chem. 298, 102467-102467 (2022).
PubMed: 36087839

Assembly members:

Assembly members:
entity_1, polymer, 28 residues, 3647.324 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ADKRCYRMCQRYHQRRQRRQ CKKGCRYG

Data sets:
Data typeCount
13C chemical shifts66
15N chemical shifts35
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 28 residues - 3647.324 Da.

1   ALAASPLYSARGCYSTYRARGMETCYSGLN
2   ARGTYRHISGLNARGARGGLNARGARGGLN
3   CYSLYSLYSGLYCYSARGTYRGLY

Samples:

sample_1: Tk-hefu-11 1 mM; sodium azide 0.0001%; sodium chloride 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1anisotropicsample_conditions_1
2D TOCSYsample_1anisotropicsample_conditions_1
3D 1H-15N TOCSYsample_1anisotropicsample_conditions_1
3D 1H-15N NOESYsample_1anisotropicsample_conditions_1
3D HNHAsample_1anisotropicsample_conditions_1
3D HNHBsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1anisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1anisotropicsample_conditions_1

Software:

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

TopSpin, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks