BMRB Entry 34702

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34702
MolProbity Validation Chart

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Title:
Red1-Iss10 complex
Deposition date:
2022-01-18
Original release date:
2022-09-02
Authors:
Mackereth, C.; Kadlec, J.; Laroussi, H.
Citation:

Citation: Foucher, Anne-Emmanuelle; Touat-Todeschini, Leila; Juarez-Martinez, Ariadna; Rakitch, Auriane; Laroussi, Hamida; Karczewski, Claire; Acajjaoui, Samira; Soler-Lopez, Montserrat; Cusack, Stephen; Mackereth, Cameron; Verdel, Andre; Kadlec, Jan. "Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex"  Nat. Commun. 13, 4969-4969 (2022).
PubMed: 36002457

Assembly members:

Assembly members:
entity_1, polymer, 55 residues, 6592.497 Da.
entity_2, polymer, 49 residues, 5299.981 Da.

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGWSHPQFEKSSDAVEPSVE KEYKKIISFRDTVFEGKHQQ FLVPNNVRLKFLRDR
entity_2: GAMGTTNQKEAEKAVSQLFE VGVRFNDFIAEGIEPSVVHT LFLKLGLDS

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts88
1H chemical shifts650

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 55 residues - 6592.497 Da.

1   METGLYTRPSERHISPROGLNPHEGLULYS
2   SERSERASPALAVALGLUPROSERVALGLU
3   LYSGLUTYRLYSLYSILEILESERPHEARG
4   ASPTHRVALPHEGLUGLYLYSHISGLNGLN
5   PHELEUVALPROASNASNVALARGLEULYS
6   PHELEUARGASPARG

Entity 2, unit_2 49 residues - 5299.981 Da.

1   GLYALAMETGLYTHRTHRASNGLNLYSGLU
2   ALAGLULYSALAVALSERGLNLEUPHEGLU
3   VALGLYVALARGPHEASNASPPHEILEALA
4   GLUGLYILEGLUPROSERVALVALHISTHR
5   LEUPHELEULYSLEUGLYLEUASPSER

Samples:

sample_1: Red1, [U-99% 15N], 150 uM; Iss10, [U-99% 15N], 150 uM; TRIS 20 mM; sodium chloride 150 mM; beta-mercaptoethanol 5 mM

sample_2: Red1, [U-99% 13C; U-99% 15N], 500 uM; Iss10, [U-99% 13C; U-99% 15N], 500 uM; TRIS 20 mM; sodium chloride 150 mM; beta-mercaptoethanol 5 mM

sample_3: Red1, [U-99% 13C; U-99% 15N], 500 uM; Iss10, [U-99% 13C; U-99% 15N], 500 uM; TRIS 20 mM; sodium chloride 150 mM; beta-mercaptoethanol 5 mM

sample_4: Red1, [U-99% 13C; U-99% 15N], 150 uM; Iss10, [U-99% 13C; U-99% 15N], 150 uM; TRIS 20 mM; sodium chloride 150 mM; beta-mercaptoethanol 5 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HA(CACO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D DQF-COSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
2D TROSY-RDC isotropicsample_1isotropicsample_conditions_1
2D TROSY-RDC phagesample_1anisotropicsample_conditions_1

Software:

TopSpin v4.0.6, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky vNMRFAM, Goddard - chemical shift assignment

ARIA v2.3, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz
  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks