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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34692
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Moursy, Ahmed; Clery, Antoine; Gerhardy, Stefan; Betz, Katharina; Rao, Sanjana; Mazur, Jaroslaw; Campagne, Sebastien; Beusch, Irene; Duszczyk, Malgorzata; Robinson, Mark; Panse, Vikram Govind; Allain, Frederic H-T. "RNA recognition by Npl3p reveals U2 snRNA-binding compatible with a chaperone role during splicing" Nat. Commun. 14, 7166-7166 (2023).
PubMed: 37935663
Assembly members:
entity_1, polymer, 11 residues, 3530.178 Da.
entity_2, polymer, 169 residues, 19294.615 Da.
Natural source: Common Name: Baker's yeast Taxonomy ID: 559292 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: unidentified plasmid
Data type | Count |
13C chemical shifts | 356 |
15N chemical shifts | 146 |
1H chemical shifts | 618 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
2 | unit_2 | 2 |
Entity 1, unit_1 11 residues - 3530.178 Da.
1 | A | U | C | C | A | G | U | G | G | A | ||||
2 | A |
Entity 2, unit_2 169 residues - 19294.615 Da.
1 | MET | HIS | HIS | ARG | GLN | GLU | GLY | GLU | LEU | SER | ||||
2 | ASN | THR | ARG | LEU | PHE | VAL | ARG | PRO | PHE | PRO | ||||
3 | LEU | ASP | VAL | GLN | GLU | SER | GLU | LEU | ASN | GLU | ||||
4 | ILE | PHE | GLY | PRO | PHE | GLY | PRO | MET | LYS | GLU | ||||
5 | VAL | LYS | ILE | LEU | ASN | GLY | PHE | ALA | PHE | VAL | ||||
6 | GLU | PHE | GLU | GLU | ALA | GLU | SER | ALA | ALA | LYS | ||||
7 | ALA | ILE | GLU | GLU | VAL | HIS | GLY | LYS | SER | PHE | ||||
8 | ALA | ASN | GLN | PRO | LEU | GLU | VAL | VAL | TYR | SER | ||||
9 | LYS | LEU | PRO | ALA | LYS | ARG | TYR | ARG | ILE | THR | ||||
10 | MET | LYS | ASN | LEU | PRO | GLU | GLY | CYS | SER | TRP | ||||
11 | GLN | ASP | LEU | LYS | ASP | LEU | ALA | ARG | GLU | ASN | ||||
12 | SER | LEU | GLU | THR | THR | PHE | SER | SER | VAL | ASN | ||||
13 | THR | ARG | ASP | PHE | ASP | GLY | THR | GLY | ALA | LEU | ||||
14 | GLU | PHE | PRO | SER | GLU | GLU | ILE | LEU | VAL | GLU | ||||
15 | ALA | LEU | GLU | ARG | LEU | ASN | ASN | ILE | GLU | PHE | ||||
16 | ARG | GLY | SER | VAL | ILE | THR | VAL | GLU | ARG | ASP | ||||
17 | ASP | ASN | PRO | PRO | PRO | ILE | ARG | ARG | SER |
sample_1: Npl3 RRM12, [U-15N], 0.9 mM
sample_2: Npl3 RRM12, [U-100% 13C; U-100% 15N], 0.9 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 0 Pa; temperature: 313 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aromatic | sample_2 | isotropic | sample_conditions_1 |
2D NOESY | sample_1 | isotropic | sample_conditions_1 |
2D NOESY F1F2f | sample_2 | isotropic | sample_conditions_1 |
2D NOESY F2f | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - peak picking, structure calculation
Sparky, Goddard - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks