BMRB Entry 34686

Name: Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b
Published: 2022-03-28

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PDB ID: 7qby
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34686
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b

Deposition date:

2021-11-21

Original release date:

2022-03-28

Authors:

Karamanos, T.; Cawood, E.

Citation:

Citation: Cawood, E.; Clore, G.; Karamanos, T.. "Microsecond Backbone Motions Modulate the Oligomerization of the DNAJB6 Chaperone." Angew. Chem. Int. Ed. Engl. 61, e202116403-e202116403 (2022).
PubMed: 35247211

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, 6444.378 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGNFKSISASTKMVNGRKIT TKRIVENGQERVEVEEDGQL KSLTINGKEQLLRLDNK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	101
15N chemical shifts	50
1H chemical shifts	53

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 57 residues - 6444.378 Da.

1

MET

GLY

ASN

PHE

LYS

SER

ILE

SER

ALA

SER

2

THR

LYS

MET

VAL

ASN

GLY

ARG

LYS

ILE

THR

3

THR

LYS

ARG

ILE

VAL

GLU

ASN

GLY

GLN

GLU

4

ARG

VAL

GLU

VAL

GLU

ASP

GLY

GLN

LEU

5

LYS

SER

LEU

THR

ILE

ASN

GLY

LYS

GLU

GLN

6

LEU

ARG

LEU

ASP

ASN

LYS

Samples:

sample_1: DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, [U-13C; U-15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02 % v/v

sample_conditions_1: ionic strength: 87 mM; pH: 6.7; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY HCH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY CCH	sample_1	isotropic	sample_conditions_1
JHNHA ARTSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr Analysis, CCPN - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz