BMRB Entry 34686

Title:
Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b
Deposition date:
2021-11-21
Original release date:
2022-03-28
Authors:
Karamanos, T.; Cawood, E.
Citation:

Citation: Cawood, E.; Clore, G.; Karamanos, T.. "Microsecond Backbone Motions Modulate the Oligomerization of the DNAJB6 Chaperone."  Angew. Chem. Int. Ed. Engl. 61, e202116403-e202116403 (2022).
PubMed: 35247211

Assembly members:

Assembly members:
entity_1, polymer, 57 residues, 6444.378 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts50
1H chemical shifts53

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 57 residues - 6444.378 Da.

1   METGLYASNPHELYSSERILESERALASER
2   THRLYSMETVALASNGLYARGLYSILETHR
3   THRLYSARGILEVALGLUASNGLYGLNGLU
4   ARGVALGLUVALGLUGLUASPGLYGLNLEU
5   LYSSERLEUTHRILEASNGLYLYSGLUGLN
6   LEULEUARGLEUASPASNLYS

Samples:

sample_1: DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, [U-13C; U-15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 150 mM; sodium azide 0.02 % v/v

sample_conditions_1: ionic strength: 87 mM; pH: 6.7; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESY HCHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY CCHsample_1isotropicsample_conditions_1
JHNHA ARTSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz