BMRB Entry 34671

Title:
Structure of Viscotoxin A3 from Viscum Album in the complex with DPC micelles
Deposition date:
2021-10-01
Original release date:
2021-11-28
Authors:
Paramonov, A.; Shenkarev, Z.
Citation:

Citation: Paramonov, Alexander; Lyukmanova, Ekaterina; Tonevitsky, Alexander; Arseniev, Alexander; Shenkarev, Zakhar. "Spatial structure and oligomerization of viscotoxin A3 in detergent micelles: Implication for mechanisms of ion channel formation and membrane lysis"  Biochem. Biophys. Res. Commun. 585, 22-28 (2021).
PubMed: 34781057

Assembly members:

Assembly members:
entity_1, polymer, 46 residues, 4842.626 Da.

Natural source:

Natural source:   Common Name: European mistletoe   Taxonomy ID: 3972   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viscum album

Experimental source:

Experimental source:   Production method: .

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: KSCCPNTTGRNIYNACRLTG APRPTCAKLSGCKIISGSTC PSDYPK

Data sets:
Data typeCount
1H chemical shifts295

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 46 residues - 4842.626 Da.

1   LYSSERCYSCYSPROASNTHRTHRGLYARG
2   ASNILETYRASNALACYSARGLEUTHRGLY
3   ALAPROARGPROTHRCYSALALYSLEUSER
4   GLYCYSLYSILEILESERGLYSERTHRCYS
5   PROSERASPTYRPROLYS

Samples:

sample_1: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM

sample_2: VtA3 1.6 ± 0.1 mM; DPC, [U-100% 2H], 128 ± 1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 4.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v3.98.13, Guntert, Mumenthaler and Wuthrich - structure calculation

XEASY v3.98.13, Bartels et al. - chemical shift assignment

CARA, Keller and Wuthrich - data analysis

XEASY, Bartels et al. - peak picking

VNMR, Varian - processing

NMR spectrometers:

  • Varian UNITY 600 MHz