BMRB Entry 34669

Title:
Solution structure of the NRDI domain of Nab3
Deposition date:
2021-09-21
Original release date:
2022-01-07
Authors:
Chaves-Arquero, B.; Martinez-Lumbreras, S.; Perez-Canadillas, J.
Citation:

Citation: Chaves-Arquero, B.; Martinez-Lumbreras, S.; Camero, S.; Santiveri, C.; Mirassou, Y.; Campos-Olivas, R.; Jimenez, M.; Calvo, O.; Perez-Canadillas, J.. "Structural basis of Nrd1-Nab3 heterodimerization"  Life Sci. Alliance 5, e202101252-e202101252 (2022).
PubMed: 35022249

Assembly members:

Assembly members:
entity_1, polymer, 53 residues, 6106.913 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts217
15N chemical shifts52
1H chemical shifts342

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 53 residues - 6106.913 Da.

1   GLYSERTRPGLYSERMETGLUASNVALASN
2   TYRASPLEULEUGLNLYSGLNVALLYSTYR
3   ILEMETASPSERASNMETLEUASNLEUPRO
4   GLNPHEGLNHISLEUPROGLNGLUGLULYS
5   METSERALAILELEUALAMETLEUASNSER
6   ASNSERASP

Samples:

sample_1: potassium phosphate 25 mM; sodium chloride 25 mM; DTT 1 mM; Nab3, [U-100% 13C; U-100% 15N], 480 uM

sample_2: potassium phosphate 25 mM; sodium chloride 25 mM; DTT 1 mM; Nab3 480 uM

sample_3: potassium phosphate 25 mM; sodium chloride 25 mM; DTT 1 mM; Nab3 480 uM

sample_4: potassium phosphate 25 mM; sodium chloride 25 mM; DTT 1 mM; Nab3, [U-100% 13C; U-100% 15N], 480 uM

sample_conditions_1: ionic strength: 53.5 mM; pH: 6.6; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr Analysis, CCPN - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks