BMRB Entry 34631

Title:
G-quadruplex structure of the C. elegans telomeric repeat: A two tetrads basket type conformation stabilised by a Hoogsteen C-T base-pair
Deposition date:
2021-06-04
Original release date:
2022-06-14
Authors:
Marquevielle, J.; De Rache, A.; Amrane, S.
Citation:

Citation: Marquevielle, J.; De Rache, A.; Vialet, B.; Morvan, E.; Mergny, J.; Amrane, S.. "G-quadruplex structure of the C. elegans telomeric repeat: a two tetrads basket type conformation stabilized by a non-canonical C-T base-pair"  Nucleic Acids Res. 50, 7134-7146 (2022).
PubMed: 35736226

Assembly members:

Assembly members:
entity_1, polymer, 20 residues, 6221.012 Da.
entity_K, non-polymer, 39.098 Da.

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGCTTAGGCTTAGGCTTAGG

Data sets:
Data typeCount
13C chemical shifts69
1H chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 20 residues - 6221.012 Da.

1   DGDGDCDTDTDADGDGDCDT
2   DTDADGDGDCDTDTDADGDG

Entity 2, unit_2 - K - 39.098 Da.

1   K

Samples:

sample_1: Asc20 2.4 mM; KCl 70 mM; KPi 20 mM

sample_conditions_1: ionic strength: 90 mM; pH: 6.9; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1
2D 1H-13C HMBCsample_1anisotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

Sparky, Goddard - chemical shift assignment

ARIA, Linge, O'Donoghue and Nilges - structure calculation

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker AVANCE III 700 MHz