BMRB Entry 34627

Name: Solution structure of Legionella pneumophila LspC
Published: 2022-05-26

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PDB ID: 7oio
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34627
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Legionella pneumophila LspC

Deposition date:

2021-05-12

Original release date:

2022-05-26

Authors:

Portlock, T.; Garnett, J.

Citation:

Citation: Portlock, T.; Garnett, J.. "Solution structure of Legionella pneumophila LspC" .

Assembly members:

Assembly members:
entity_1, polymer, 144 residues, 15995.113 Da.

Natural source:

Natural source: Common Name: Legionella pneumophila Taxonomy ID: 446 Superkingdom: Bacteria Kingdom: not available Genus/species: Legionella pneumophila

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHHHHHHVDDDDKMTYVRA IPVSNTKSIAGMREENLNYI FNTSLFGVYVPADLNEDNVK QSMLNVTLVGILFADKIEES QVIIRSASGEEKTYNVGDKI PGGATIKRIMPGGVLVERDG TLESLSLPKNDLTFEPVAKP LKEE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	529
15N chemical shifts	115
1H chemical shifts	840

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 144 residues - 15995.113 Da.

1

MET

ALA

HIS

VAL

ASP

2

ASP

LYS

MET

THR

TYR

VAL

ARG

ALA

3

ILE

PRO

VAL

SER

ASN

THR

LYS

SER

ILE

ALA

4

GLY

MET

ARG

GLU

ASN

LEU

ASN

TYR

ILE

5

PHE

ASN

THR

SER

LEU

PHE

GLY

VAL

TYR

VAL

6

PRO

ALA

ASP

LEU

ASN

GLU

ASP

ASN

VAL

LYS

7

GLN

SER

MET

LEU

ASN

VAL

THR

LEU

VAL

GLY

8

ILE

LEU

PHE

ALA

ASP

LYS

ILE

GLU

SER

9

GLN

VAL

ILE

ARG

SER

ALA

SER

GLY

GLU

10

GLU

LYS

THR

TYR

ASN

VAL

GLY

ASP

LYS

ILE

11

PRO

GLY

ALA

THR

ILE

LYS

ARG

ILE

MET

12

PRO

GLY

VAL

LEU

VAL

GLU

ARG

ASP

GLY

13

THR

LEU

GLU

SER

LEU

SER

LEU

PRO

LYS

ASN

14

ASP

LEU

THR

PHE

GLU

PRO

VAL

ALA

LYS

PRO

15

LEU

LYS

GLU

Samples:

sample_1: LspC-PER, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks