BMRB Entry 34598

Name: Tick salivary protein BSAP1
Published: 2021-06-18

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PDB ID: 7ne8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34598
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Tick salivary protein BSAP1

Deposition date:

2021-02-03

Original release date:

2021-06-18

Authors:

Denisov, S.; Ippel, J.; Castoldi, E.; Hackeng, T.; Dijkgraaf, I.

Citation:

Citation: Denisov, Stepan; Ippel, Johannes; Castoldi, Elisabetta; Mans, Ben; Hackeng, Tilman; Dijkgraaf, Ingrid. "The basic tail of the tick salivary protein Salp14 inhibits early stages of blood coagulation and the lectin complement pathway." J. Biol. Chem. 297, 100865-100865 (2021).
PubMed: 34118237

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, 9634.224 Da.

Natural source:

Natural source: Common Name: Ornithodoros savignyi Taxonomy ID: 69826 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ornithodoros savignyi

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DSEFPCPRKQQPAGNSECSY YCEMNGQWKLGKFQNGARCD YNAVKDGVCNEGLCYASGDS ASNTQNQGGSRRQENEDQGD DEWDRK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	241
15N chemical shifts	98
1H chemical shifts	521

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 86 residues - 9634.224 Da.

1

ASP

SER

GLU

PHE

PRO

CYS

PRO

ARG

LYS

GLN

2

GLN

PRO

ALA

GLY

ASN

SER

GLU

CYS

SER

TYR

3

TYR

CYS

GLU

MET

ASN

GLY

GLN

TRP

LYS

LEU

4

GLY

LYS

PHE

GLN

ASN

GLY

ALA

ARG

CYS

ASP

5

TYR

ASN

ALA

VAL

LYS

ASP

GLY

VAL

CYS

ASN

6

GLU

GLY

LEU

CYS

TYR

ALA

SER

GLY

ASP

SER

7

ALA

SER

ASN

THR

GLN

ASN

GLN

GLY

SER

8

ARG

GLN

GLU

ASN

GLU

ASP

GLN

GLY

ASP

9

ASP

GLU

TRP

ASP

ARG

LYS

Samples:

sample_1: tick salivary protein BSAP1, [U-13C; U-15N], 350 uM

sample_conditions_1: ionic strength: 20 mM; pH: 7.1; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY, Lee W, Tonelli M, Markley JL - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

Bruker AVANCE III HD 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks