BMRB Entry 34584

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PDB ID: 7bbb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34584
MolProbity Validation Chart

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Title:
Solution structure of C-terminal RecA and RRM domains of the DEAD box helicase DbpA
Deposition date:
2020-12-17
Original release date:
2021-07-12
Authors:
Wurm, J.; Sprangers, R.
Citation:

Citation: Wurm, Jan Philip; Glowacz, Katarzyna-Anna; Sprangers, Remco. "Structural basis for the activation of the DEAD-box RNA helicase DbpA by the nascent ribosome"  Proc. Natl. Acad. Sci. U. S. A. 118, e2105961118-e2105961118 (2021).
PubMed: 34453003

Assembly members:

Assembly members:
entity_1, polymer, 251 residues, 27014.055 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSTDALPPIEQQFYETSSK GKIPLLQRLLSLHQPSSCVV FCNTKKDCQAVCDALNEVGQ SALSLHGDLEQRDRDQTLVR FANGSARVLVATDVAARGLD IKSLELVVNFELAWDPEVHV HRIGRTARAGNSGLAISFCA PEEAQRANIISDMLQIKLNW QTPPANSSIATLEAEMATLC IDGGKKAKMRPGDVLGALTG DIGLDGADIGKIAVHPAHVY VAVRQAVAHKAWKQLQGGKI KGKTCRVRLLK

Data sets:
Data typeCount
13C chemical shifts804
15N chemical shifts251
1H chemical shifts738

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 251 residues - 27014.055 Da.

1   GLYGLYSERTHRASPALALEUPROPROILE
2   GLUGLNGLNPHETYRGLUTHRSERSERLYS
3   GLYLYSILEPROLEULEUGLNARGLEULEU
4   SERLEUHISGLNPROSERSERCYSVALVAL
5   PHECYSASNTHRLYSLYSASPCYSGLNALA
6   VALCYSASPALALEUASNGLUVALGLYGLN
7   SERALALEUSERLEUHISGLYASPLEUGLU
8   GLNARGASPARGASPGLNTHRLEUVALARG
9   PHEALAASNGLYSERALAARGVALLEUVAL
10   ALATHRASPVALALAALAARGGLYLEUASP
11   ILELYSSERLEUGLULEUVALVALASNPHE
12   GLULEUALATRPASPPROGLUVALHISVAL
13   HISARGILEGLYARGTHRALAARGALAGLY
14   ASNSERGLYLEUALAILESERPHECYSALA
15   PROGLUGLUALAGLNARGALAASNILEILE
16   SERASPMETLEUGLNILELYSLEUASNTRP
17   GLNTHRPROPROALAASNSERSERILEALA
18   THRLEUGLUALAGLUMETALATHRLEUCYS
19   ILEASPGLYGLYLYSLYSALALYSMETARG
20   PROGLYASPVALLEUGLYALALEUTHRGLY
21   ASPILEGLYLEUASPGLYALAASPILEGLY
22   LYSILEALAVALHISPROALAHISVALTYR
23   VALALAVALARGGLNALAVALALAHISLYS
24   ALATRPLYSGLNLEUGLNGLYGLYLYSILE
25   LYSGLYLYSTHRCYSARGVALARGLEULEU
26   LYS

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