BMRB Entry 34571

Name: G-quadruplex with V-shaped loop from the first repeat of KCNN4 minisatellite
Published: 2021-04-13

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PDB ID: 7atz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34571
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

G-quadruplex with V-shaped loop from the first repeat of KCNN4 minisatellite

Deposition date:

2020-11-02

Original release date:

2021-04-13

Authors:

Vianney, Y.; Weisz, K.

Citation:

Citation: Vianney, Y.; Weisz, K.. "First Tandem Repeat of a Potassium Channel KCNN4 Minisatellite Folds into a V-Loop G-Quadruplex Structure" Biochemistry 60, 1337-1346 (2021).
PubMed: 33844501

Assembly members:

Assembly members:
DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3'), polymer, 23 residues, 7298.680 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA (5'-D(*GP*GP*TP*CP*TP*GP*AP*GP*GP*GP*AP*GP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*T)-3'): GGTCTGAGGGAGAGGGGCTG GGT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	26
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 23 residues - 7298.680 Da.

1

DG

DT

DC

DT

DG

DA

DG

2

DA

DG

DA

DG

DC

DT

DG

3

DG

DT

Samples:

sample_1: DNA 0.9 mM

sample_2: DNA 0.9 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMBC	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, data analysis

TopSpin v4.0.7, Bruker Biospin - processing

X-PLOR NIH v3.0.3, Schwieters, Kuszewski, Tjandra and Clore - refinement

Amber v16, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker AVANCE NEO 600 MHz