BMRB Entry 34560

Title:
Solution structure of U1-A RRM2 (190-282)
Deposition date:
2020-09-18
Original release date:
2021-02-01
Authors:
Campagne, S.; Allain, F.
Citation:

Citation: Campagne, S.; Allain, F.. "Solution structure of U1-A RRM2 (190-282)"  .

Assembly members:

Assembly members:
entity_1, polymer, 126 residues, 13775.973 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Data typeCount
13C chemical shifts492
15N chemical shifts112
1H chemical shifts718

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 126 residues - 13775.973 Da.

1   METPROGLYGLNPROPROTYRMETPROPRO
2   PROGLYMETILEPROPROPROGLYLEUALA
3   PROGLYGLNILEPROPROGLYALAMETPRO
4   PROGLNGLNLEUMETPROGLYGLNMETPRO
5   PROALAGLNPROLEUSERGLUASNPROPRO
6   ASNHISILELEUPHELEUTHRASNLEUPRO
7   GLUGLUTHRASNGLULEUMETLEUSERMET
8   LEUPHEASNGLNPHEPROGLYPHELYSGLU
9   VALARGLEUVALPROGLYARGHISASPILE
10   ALAPHEVALGLUPHEASPASNGLUVALGLN
11   ALAGLYALAALAARGASPALALEUGLNGLY
12   PHELYSILETHRGLNASNASNALAMETLYS
13   ILESERPHEALALYSLYS

Samples:

sample_1: entity_1 mM; NaPO4 10 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 60 mM; pH: 6.8; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

Amber v20, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v3.94, Guntert, Mumenthaler and Wuthrich - structure calculation

CARA v1.9, Keller and Wuthrich - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - peak picking

NMR spectrometers:

  • Bruker 600 AVIII 600 MHz
  • Bruker 900 AVII 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks