BMRB Entry 34555

Name: Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2
Published: 2021-10-01

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PDB ID: 7aaf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34555
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2

Deposition date:

2020-09-04

Original release date:

2021-10-01

Authors:

Kawale, A.; Kang, H.; Sattler, M.

Citation:

Citation: Kawale, A.; Kang, H.; Sattler, M.. "Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2" .

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 8907.245 Da.

Natural source:

Natural source: Common Name: Fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AMGNKIYVGGLPTCLNQDQV KELLQSFGELKGLNLVMDTN TNLNKGFAFFEYCDPSVTDH AIAGLHGMLLGDRRLVVQRS I

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	269
15N chemical shifts	87
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 81 residues - 8907.245 Da.

1

ALA

MET

GLY

ASN

LYS

ILE

TYR

VAL

GLY

2

LEU

PRO

THR

CYS

LEU

ASN

GLN

ASP

GLN

VAL

3

LYS

GLU

LEU

GLN

SER

PHE

GLY

GLU

LEU

4

LYS

GLY

LEU

ASN

LEU

VAL

MET

ASP

THR

ASN

5

THR

ASN

LEU

ASN

LYS

GLY

PHE

ALA

PHE

6

GLU

TYR

CYS

ASP

PRO

SER

VAL

THR

ASP

HIS

7

ALA

ILE

ALA

GLY

LEU

HIS

GLY

MET

LEU

8

GLY

ASP

ARG

LEU

VAL

GLN

ARG

SER

9

ILE

Samples:

sample_1: LS2 RRM2, [U-13C; U-15N], 0.5 mM; potassium phosphate pH 6.5 0.02 mM; sodium chloride 0.05 mM; DTT 0.002 mM

sample_conditions_1: ionic strength: 0.07 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE 750 750 MHz
Bruker AVANCE 800 800 MHz
Bruker AVANCE 900 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks