BMRB Entry 34544

Title:
Solution structure of the C-terminal domain of the vaccinia virus DNA polymerase processivity factor component A20 fused to a short peptide from the viral DNA polymerase E9.
Deposition date:
2020-07-30
Original release date:
2021-05-12
Authors:
Bersch, B.; Tarbouriech, N.; Burmeister, W.; Iseni, F.
Citation:

Citation: Bersch, B.; Tarbouriech, N.; Burmeister, W.; Iseni, F.. "Solution structure of the C-terminal domain of A20, the missing brick for the characterization of the interface between vaccinia virus DNA polymerase and its processivity factor"  J. Mol. Biol. 433, 167009-167009 (2021).
PubMed: 33901538

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, 16882.934 Da.

Natural source:

Natural source:   Common Name: VACV   Taxonomy ID: 10249   Superkingdom: Viruses   Kingdom: not available   Genus/species: Orthopoxvirus VACV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data typeCount
13C chemical shifts583
15N chemical shifts169
1H chemical shifts1008
T1 relaxation values116
T2 relaxation values116
heteronuclear NOE values118

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 151 residues - 16882.934 Da.

1   GLYASNGLYLYSTYRPHESERLYSVALGLY
2   SERALAGLYLEULYSGLNLEUTHRASNLYS
3   LEUASPILEASNGLUCYSALATHRVALASP
4   GLULEUVALASPGLUILEASNLYSSERGLY
5   THRVALLYSARGLYSILELYSASNGLNSER
6   ALAPHEASPLEUSERARGGLUCYSLEUGLY
7   TYRPROGLUALAASPPHEILETHRLEUVAL
8   ASNASNMETARGPHELYSILEGLUASNCYS
9   LYSVALVALASNPHEASNILEGLUASNTHR
10   ASNCYSLEUASNASNPROSERILEGLUTHR
11   ILETYRARGASNPHEASNGLNPHEVALSER
12   ILEPHEASNVALVALTHRASPVALLYSLYS
13   ARGLEUPHEGLUASNALASERGLYASNGLY
14   SERGLYGLYGLYSERASNARGLEUGLUGLU
15   GLUILEASNASNGLNLEUALALEUGLNLYS
16   SER

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