BMRB Entry 34523

Name: Solution NMR structure of human GATA2 N-terminal zinc finger domain
Published: 2021-06-25

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PDB ID: 6zfv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34523
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of human GATA2 N-terminal zinc finger domain

Deposition date:

2020-06-18

Original release date:

2021-06-25

Authors:

Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.

Citation:

Citation: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.. "Solution NMR structure of human GATA2 C-terminal zinc finger domain" .

Assembly members:

Assembly members:
entity_1, polymer, 63 residues, 7060.018 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHHHHHHSSGLEVLFQGPE GRECVNCGATATPLWRRDGT GHYLCNACGLYHKMNGQNRP LIK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	131
15N chemical shifts	53
1H chemical shifts	290

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2

Entities:

Entity 1, unit_1 63 residues - 7060.018 Da.

1

MET

ALA

HIS

SER

2

GLY

LEU

GLU

VAL

LEU

PHE

GLN

GLY

PRO

GLU

3

GLY

ARG

GLU

CYS

VAL

ASN

CYS

GLY

ALA

THR

4

ALA

THR

PRO

LEU

TRP

ARG

ASP

GLY

THR

5

GLY

HIS

TYR

LEU

CYS

ASN

ALA

CYS

GLY

LEU

6

TYR

HIS

LYS

MET

ASN

GLY

GLN

ASN

ARG

PRO

7

LEU

ILE

LYS

Entity 2, unit_2 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: GATA2 zinc finger 1, [U-99% 13C; U-99% 15N], 10.0 mg/mL

sample_conditions_1: ionic strength: 150 mM; pH: 5.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement

CcpNmr Analysis v2.4.1, CCPN - chemical shift assignment

NMR spectrometers:

Bruker DRX500 500 MHz
Bruker DRX800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks