BMRB Entry 34483

Title:
Constitutive decay element CDE1 from human 3'UTR
Deposition date:
2020-01-27
Original release date:
2020-05-25
Authors:
Schwalbe, H.; Binas, O.
Citation:

Citation: Binas, O.; Tants, J.; Peter, S.; Janowski, R.; Davydova, E.; Braun, J.; Niessing, D.; Schwalbe, H.; Weigand, J.; Schlundt, A.. "Structural basis for the recognition of transiently structured AU-rich elements by Roquin"  Nucleic Acids Res. ., .-. (2020).

Assembly members:

Assembly members:
entity_1, polymer, 19 residues, 6009.584 Da.

Natural source:

Natural source:   Common Name: Thermosinus carboxydivorans Nor1   Taxonomy ID: 401526   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosinus carboxydivorans

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Thermosinus carboxydivorans Nor1   Taxonomy ID: 401526   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosinus carboxydivorans

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGAAAUUAUAUUAAUUUCC

Data sets:
Data typeCount
13C chemical shifts112
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 19 residues - 6009.584 Da.

1   GGAAAUUAUA
2   UUAAUUUCC

Samples:

sample_1: CDE1 350 uM; KCl 50 mM; potassium phosphate buffer 25 mM

sample_2: CDE1, [U-99% 13C; U-99% 15N], 300 uM; KCl 50 mM; potassium phosphate buffer 25 mM

sample_conditions_1: ionic strength: 75 mM; pH: 6.2; pressure: 1 bar; temperature: 298 K

sample_conditions_2: ionic strength: 75 mM; pH: 6.2; pressure: 1 bar; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
fwHCC-TOCSY-CCH-E.COSYsample_2isotropicsample_conditions_2
3D HCPsample_2isotropicsample_conditions_2
3D HCNsample_2isotropicsample_conditions_2
2D gamma HCNsample_2isotropicsample_conditions_2
2D PFIDSsample_2isotropicsample_conditions_2

Software:

CNS v1.2, Brunger A. T. et.al. - structure calculation

Sparky v3.2, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 800 MHz
  • Bruker AVANCE III 600 MHz
  • Bruker AVANCE III 700 MHz