BMRB Entry 34462

Title:
Solution structure of the modulator of repression (MOR) of the temperate bacteriophage TP901-1 from Lactococcus lactis
Deposition date:
2019-12-11
Original release date:
2020-08-03
Authors:
Rasmussen, K.; Blackledge, M.; Herrmann, T.; Lo Leggio, L.; Jensen, M.
Citation:

Citation: Rasmussen, K.; Blackledge, M.; Herrmann, T.; Lo Leggio, L.; Jensen, M.. "Solution structure of the modulator of repression (MOR) of the temperate bacteriophage TP901-1 from Lactococcus lactis"  .

Assembly members:

Assembly members:
entity_1, polymer, 74 residues, 8422.644 Da.

Natural source:

Natural source:   Common Name: Lactococcus phage TP901-1   Taxonomy ID: 35345   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available Lactococcus phage TP901-1

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Experimental source:

Natural source:   Common Name: Lactococcus phage TP901-1   Taxonomy ID: 35345   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available Lactococcus phage TP901-1

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts76
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 74 residues - 8422.644 Da.

1   GLYSERMETSERTYRASPTYRSERSERLEU
2   LEUGLYLYSILETHRGLULYSCYSGLYTHR
3   GLNTYRASNPHEALAILEALAMETGLYLEU
4   SERGLUARGTHRVALSERLEULYSLEUASN
5   ASPLYSVALTHRTRPLYSASPASPGLUILE
6   LEULYSALAVALHISVALLEUGLULEUASN
7   PROGLNASPILEPROLYSTYRPHEPHEASN
8   ALALYSVALHIS

Samples:

sample_1: MOR, [U-13C; U-15N], 700 uM; Tris buffer 20 mM; NaCl 100 mM; dithiothreitol (DTT) 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HCCH-TOCSYsample_1isotropicsample_conditions_1
[1H-1H] NOESY-15N-HSQCsample_1isotropicsample_conditions_1
aliphatic [1H-1H]-NOESY-13C-HSQCsample_1isotropicsample_conditions_1
aromatic [1H-1H]-NOESY-13C-HSQCsample_1isotropicsample_conditions_1
HNCOsample_1isotropicsample_conditions_1
HN(CA)COsample_1isotropicsample_conditions_1
HN(CO)CACBsample_1isotropicsample_conditions_1
HNCACBsample_1isotropicsample_conditions_1
HSQCsample_1isotropicsample_conditions_1

Software:

UNIO, Herrmann, T. et al - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CcpNmr Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 950 MHz
  • Bruker AVANCE III 700 MHz
  • Bruker AVANCE III 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts