BMRB Entry 34400

Name: RW16 peptide
Published: 2019-10-21

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PDB ID: 6rqs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34400
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RW16 peptide

Deposition date:

2019-05-16

Original release date:

2019-10-21

Authors:

Jobin, M.; Grelard, A.; Alves, I.; Mackereth, C.

Citation:

Citation: Jobin, M.; Vamparys, L.; Deniau, R.; Grelard, A.; Mackereth, C.; Fuchs, P.; Alves, I.. "Biophysical Insight on the Membrane Insertion of an Arginine-Rich Cell-Penetrating Peptide." Int. J. Mol. Sci. 20, E4441-E4441 (2019).
PubMed: 31505894

Assembly members:

Assembly members:
entity_1, polymer, 18 residues, 3032.639 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: synthetic construct

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XXRRWRRWWRRWWRRWRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
13C chemical shifts	30
1H chemical shifts	146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 18 residues - 3032.639 Da.

1

BTN

KFB

ARG

TRP

ARG

TRP

ARG

2

ARG

TRP

ARG

TRP

ARG

Samples:

sample_1: RW16 peptide 1 mM; DPC, [U-100% 2H], 60 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TopSpin v3.2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky v3.113, Goddard - data analysis

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

ARIA v1.2, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

Bruker AVANCE III 800 MHz