BMRB Entry 34380

Name: Structure of peptide P7, which binds Cdc42 and inhibits effector interactions.
Published: 2020-01-24

Click here to enlarge.

PDB ID: 6r28
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34380
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Structure of peptide P7, which binds Cdc42 and inhibits effector interactions.

Deposition date:

2019-03-15

Original release date:

2020-01-24

Authors:

Murphy, N.; Mott, H.; Owen, D.

Citation:

Citation: Tetley, G.; Murphy, N.; Bonetto, S.; Ivanova, G.; Revell, J.; Mott, H.; Cooley, R.; Owen, D.. "The discovery and maturation of peptide biologics targeting the small G protein Cdc42: a bioblockade for Ras-driven signalling" J. Biol. Chem. 295, 2866-2884 (2020).
PubMed: 31959628

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 2070.401 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: PSIXHVHRPDWPCWYR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list
- spectral_peak_list_1

Data type	Count
1H chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 16 residues - 2070.401 Da.

1

PRO

SER

ILE

HCS

HIS

VAL

HIS

ARG

PRO

ASP

2

TRP

PRO

CYS

TRP

TYR

ARG

Samples:

sample_1: peptide 5 mg/mL; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
TOCSY	sample_1	isotropic	sample_conditions_1
COSY	sample_1	isotropic	sample_conditions_1
NOESY	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr Analysis v2, CCPN - chemical shift assignment

ARIA v1.2, Linge, O'Donoghue and Nilges - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation

NMR spectrometers:

Bruker AVANCE III 800 MHz