BMRB Entry 34335

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34335
MolProbity Validation Chart

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Title:
H-Vc7.2, H-superfamily conotoxin
Deposition date:
2018-12-10
Original release date:
2019-04-11
Authors:
Nielsen, L.; Foged, M.; Teilum, K.; Ellgaard, L.
Citation:

Citation: Nielsen, Lau; Foged, Mads; Albert, Anastasia; Bertelsen, Andreas; Soltoft, Cecilie; Robinson, Samuel; Petersen, Steen; Purcell, Anthony; Olivera, Baldomero; Norton, Raymond; Vasskog, Terje; Safavi-Hemami, Helena; Teilum, Kaare; Ellgaard, Lars. "The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework"  J. Biol. Chem. 294, 8745-8759 (2019).
PubMed: 30975904

Assembly members:

Assembly members:
entity_1, polymer, 29 residues, 3112.570 Da.

Natural source:

Natural source:   Common Name: Queen Victoria cone   Taxonomy ID: 319920   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus victoriae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGNVNCGGVPCKFGCCRE DRCREIDCD

Data sets:
Data typeCount
13C chemical shifts63
15N chemical shifts22
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 29 residues - 3112.570 Da.

1   GLYALAMETGLYASNVALASNCYSGLYGLY
2   VALPROCYSLYSPHEGLYCYSCYSARGGLU
3   ASPARGCYSARGGLUILEASPCYSASP

Samples:

sample_1: H-Vc7.2 1 mM; Na-phosphate 25 mM

sample_2: H-Vc7.2, [U-13C; U-15N], 1 mM; Na-phosphate 25 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker AvanceIII 600 MHz
  • Bruker AvanceIII 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks