BMRB Entry 34298

Name: Solution structure of Melampsora larici-populina MlpP4.1
Published: 2018-08-16

Click here to enlarge.

PDB ID: 6h0i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34298
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of Melampsora larici-populina MlpP4.1

Deposition date:

2018-07-09

Original release date:

2018-08-16

Authors:

Tsan, P.

Citation:

Citation: de Guillen, K.; Lorrain, C.; Tsan, P.; Barthe, P.; Petre, B.; Saveleva, N.; Rouhier, N.; Duplessis, S.; Padilla, A.; Hecker, A.. "Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds." Sci. Rep. 9, 18084-18084 (2019).
PubMed: 31792250

Assembly members:

Assembly members:
entity_1, polymer, 75 residues, 7978.616 Da.

Natural source:

Natural source: Common Name: Melampsora larici-populina Taxonomy ID: 747676 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Melampsora larici-populina

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MCEFIEDSEDIQGLKSLRKS HTSLEDDDDGSRGGDCEGCS GTACSSDAQCRARGCDGCST SGVCVLSSLHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	115
15N chemical shifts	67
1H chemical shifts	318

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 75 residues - 7978.616 Da.

1

MET

CYS

GLU

PHE

ILE

GLU

ASP

SER

GLU

ASP

2

ILE

GLN

GLY

LEU

LYS

SER

LEU

ARG

LYS

SER

3

HIS

THR

SER

LEU

GLU

ASP

GLY

4

SER

ARG

GLY

ASP

CYS

GLU

GLY

CYS

SER

5

GLY

THR

ALA

CYS

SER

ASP

ALA

GLN

CYS

6

ARG

ALA

ARG

GLY

CYS

ASP

GLY

CYS

SER

THR

7

SER

GLY

VAL

CYS

VAL

LEU

SER

LEU

HIS

8

HIS

Samples:

sample_1: entity_1, [U-15N], 1.8 mM; potassium phosphate 50 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis

ARIA, Linge, O'Donoghue and Nilges - structure calculation

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks