BMRB Entry 34267

Name: Antinociceptive evaluation of cyriotoxin-1a, the first toxin purified from Cyriopagopus schioedtei spider venom
Published: 2019-03-14

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PDB ID: 6gft
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34267
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Antinociceptive evaluation of cyriotoxin-1a, the first toxin purified from Cyriopagopus schioedtei spider venom

Deposition date:

2018-05-02

Original release date:

2019-03-14

Authors:

Kurz, M.

Citation:

Citation: Goncalves, T.; Benoit, E.; Kurz, M.; Lucarain, L.; Fouconnier, S.; Combemale, S.; Jaquillard, L.; Schombert, B.; Chambard, J.; Boukaiba, R.; Hessler, G.; Bohme, A.; Bialy, L.; Hourcade, S.; Beroud, R.; De Waard, M.; Servent, D.; Partiseti, M.. "From identification to functional characterization of cyriotoxin-1a, an antinociceptive toxin from Cyriopagopus schioedtei spider." Br. J. Pharmacol. 176, 1298-1314 (2019).
PubMed: 30784059

Assembly members:

Assembly members:
cyriotoxin-1a, polymer, 34 residues, 3593.317 Da.

Natural source:

Natural source: Common Name: Cyriopagopus schioedtei Taxonomy ID: 1046902 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Cyriopagopus schioedtei

Experimental source:

Experimental source: Production method: na

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cyriotoxin-1a: ECKGFGKSCVPGKNECCSGL TCSNKHKWCKVLLX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	220

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 34 residues - 3593.317 Da.

1

GLU

CYS

LYS

GLY

PHE

GLY

LYS

SER

CYS

VAL

2

PRO

GLY

LYS

ASN

GLU

CYS

SER

GLY

LEU

3

THR

CYS

SER

ASN

LYS

HIS

LYS

TRP

CYS

LYS

4

VAL

LEU

NH2

Samples:

sample_1: cyriotoxin-1a, unlabeled, 6 ± 0.2 mg/mL

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 bar; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - chemical shift assignment

SYBYL v2.1.1, Tripos - structure calculation

NMR spectrometers:

Bruker Avance 700 MHz
Bruker AvanceIII 500 MHz