BMRB Entry 34249

Name: FLN5 (full length)
Published: 2019-04-03

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PDB ID: 6g4a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34249
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

FLN5 (full length)

Deposition date:

2018-03-27

Original release date:

2019-04-03

Authors:

Waudby, C.; Wlodarski, T.; Karyadi, M.; Cassaignau, A.; Chan, S.; Wentink, A.; Schmidt-Engler, J.; Camilloni, C.; Vendruscolo, M.; Cabrita, L.; Christodoulou, J.

Citation:

Citation: Waudby, C.; Wlodarski, T.; Karyadi, M.; Cassaignau, A.; Chan, S.; Wentink, A.; Schmidt-Engler, J.; Camilloni, C.; Vendruscolo, M.; Cabrita, L.; Christodoulou, J.. "Mapping energy landscapes of a growing filamin domain reveals an intermediate associated with proline isomerization during biosynthesis" .

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, 12167.383 Da.

Natural source:

Natural source: Common Name: Slime mold Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHASKPAPSAEHSYA EGEGLVKVFDNAPAEFTIFA VDTKGVARTDGGDPFEVAIN GPDGLVVDAKVTDNNDGTYG VVYDAPVEGNYNVNVTLRGN PIKNMPIDVKCIEG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	94
1H chemical shifts	196

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 114 residues - 12167.383 Da.

1

MET

HIS

ALA

SER

LYS

2

PRO

ALA

PRO

SER

ALA

GLU

HIS

SER

TYR

ALA

3

GLU

GLY

GLU

GLY

LEU

VAL

LYS

VAL

PHE

ASP

4

ASN

ALA

PRO

ALA

GLU

PHE

THR

ILE

PHE

ALA

5

VAL

ASP

THR

LYS

GLY

VAL

ALA

ARG

THR

ASP

6

GLY

ASP

PRO

PHE

GLU

VAL

ALA

ILE

ASN

7

GLY

PRO

ASP

GLY

LEU

VAL

ASP

ALA

LYS

8

VAL

THR

ASP

ASN

ASP

GLY

THR

TYR

GLY

9

VAL

TYR

ASP

ALA

PRO

VAL

GLU

GLY

ASN

10

TYR

ASN

VAL

ASN

VAL

THR

LEU

ARG

GLY

ASN

11

PRO

ILE

LYS

ASN

MET

PRO

ILE

ASP

VAL

LYS

12

CYS

ILE

GLU

GLY

Samples:

sample_1: FLN5, [U-13C; U-15N], 200 uM; HEPES 10 mM; ammonium chloride 30 mM; magnesium chloride 12 mM; beta-mercaptoethanol 2 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 158 mM; pH: 7.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment

GROMACS v4.6.5, http://www.gromacs.org/ - structure calculation

NMR spectrometers:

Bruker AvanceIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks