BMRB Entry 34220

Name: NMR Solution Structure of MINA-1(254-334)
Published: 2019-01-28

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PDB ID: 6fbl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34220
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure of MINA-1(254-334)

Deposition date:

2017-12-19

Original release date:

2019-01-28

Authors:

Michel, E.; Allain, F.

Citation:

Citation: Sendoel, Ataman; Subasic, Deni; Ducoli, Luca; Keller, Martin; Michel, Erich; Kohler, Ines; Singh, Kapil Dev; Zheng, Xue; Brummer, Anneke; Imig, Jochen; Kishore, Shivendra; Wu, Yibo; Kanitz, Alexander; Kaech, Andres; Mittal, Nitish; Matia-Gonzalez, Ana; Gerber, Andre; Zavolan, Mihaela; Aebersold, Ruedi; Hall, Jonathan; Allain, Frederic H-T; Hengartner, Michael. "MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans" Cell Death Differ. 26, 2157-2178 (2019).
PubMed: 30728462

Assembly members:

Assembly members:
entity_1, polymer, 86 residues, 9634.081 Da.

Natural source:

Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pCFX3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGSHMKTCVVEKIKQWIPTT EVGKILGNRAAVKKHIERQF NCVITVHTEVQSSFGATPVE IVAQNKEQCQEARNAVMSLM QSHQDK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	286
15N chemical shifts	98
1H chemical shifts	608

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 86 residues - 9634.081 Da.

1

SER

GLY

SER

HIS

MET

LYS

THR

CYS

VAL

2

GLU

LYS

ILE

LYS

GLN

TRP

ILE

PRO

THR

3

GLU

VAL

GLY

LYS

ILE

LEU

GLY

ASN

ARG

ALA

4

ALA

VAL

LYS

HIS

ILE

GLU

ARG

GLN

PHE

5

ASN

CYS

VAL

ILE

THR

VAL

HIS

THR

GLU

VAL

6

GLN

SER

PHE

GLY

ALA

THR

PRO

VAL

GLU

7

ILE

VAL

ALA

GLN

ASN

LYS

GLU

GLN

CYS

GLN

8

GLU

ALA

ARG

ASN

ALA

VAL

MET

SER

LEU

MET

9

GLN

SER

HIS

GLN

ASP

LYS

Samples:

sample_1: MINA-1(254-334), [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; EDTA 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

ATNOS, Herrmann, Guntert and Wuthrich - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 700 MHz
Bruker Avance 600 MHz
Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks