BMRB Entry 34151

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34151
MolProbity Validation Chart

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Title:
Solution structure of the complexed RCD1-RST
Deposition date:
2017-06-23
Original release date:
2018-04-13
Authors:
Bugge, K.; Staby, L.; Skriver, K.; Kragelund, B.
Citation:

Citation: Bugge, Katrine; Staby, Lasse; Kemplen, Katherine; O'Shea, Charlotte; Bendsen, Sidsel; Jensen, Mikael; Olsen, Johan; Skriver, Karen; Kragelund, Birthe. "Structure of Radical-Induced Cell Death1 Hub Domain Reveals a Common aa-Scaffold for Disorder in Transcriptional Networks"  Structure 26, 734-746 (2018).
PubMed: 29657132

Assembly members:

Assembly members:
RST domain from the Radical-Induced Cell Death1, polymer, 73 residues, 8455.854 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RST domain from the Radical-Induced Cell Death1: TTRPKSPWMPFPTLFAAISH KVAENDMLLINADYQQLRDK KMTRAEFVRKLRVIVGDDLL RSTITTLQNQPKS

Data sets:
Data typeCount
13C chemical shifts245
15N chemical shifts60
1H chemical shifts381

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 73 residues - 8455.854 Da.

1   THRTHRARGPROLYSSERPROTRPMETPRO
2   PHEPROTHRLEUPHEALAALAILESERHIS
3   LYSVALALAGLUASNASPMETLEULEUILE
4   ASNALAASPTYRGLNGLNLEUARGASPLYS
5   LYSMETTHRARGALAGLUPHEVALARGLYS
6   LEUARGVALILEVALGLYASPASPLEULEU
7   ARGSERTHRILETHRTHRLEUGLNASNGLN
8   PROLYSSER

Samples:

sample_1: DREB2A 200 uM; DSS 0.7 mM; RCD1-RST, [U-13C; U-15N], 200 uM; sodium azide 0.2 mg/mL; NaCl 100 mM

sample_2: DREB2A 150 uM; DSS 0.7 mM; RCD1-RST, [U-15N], 150 uM; sodium azide 0.2 mg/mL; NaCl 100 mM

sample_3: DREB2A, [U-13C; U-15N], 200 uM; DSS 0.7 mM; RCD1-RST, [U-13C; U-15N], 200 uM; sodium azide 0.2 mg/mL; NaCl 100 mM

sample_4: C12E5 50 mg/mL; DREB2A 900 uM; DSS 0.7 mM; RCD1-RST, [U-15N], 300 uM; sodium azide 0.2 mg/mL; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_3isotropicsample_conditions_1
2D 1H-15N IPAP-HSQCsample_4isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N IPAP-HSQCsample_4anisotropicsample_conditions_1

Software:

Analysis, CCPN - chemical shift assignment, data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ProcheckNMR, Laskowski and MacArthur - geometry optimization

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

VNMR, Varian - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 750 MHz
  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks