BMRB Entry 34149

Name: Solution NMR structure of human GATA2 C-terminal zinc finger domain
Published: 2019-02-01

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PDB ID: 5o9b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34149
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of human GATA2 C-terminal zinc finger domain

Deposition date:

2017-06-16

Original release date:

2019-02-01

Authors:

Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.

Citation:

Citation: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.. "Solution NMR structure of human GATA2 C-terminal zinc finger domain" .

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, 7700.792 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAHHHHHHSSGLEVLFQGPR RAGTCCANCQTTTTTLWRRN ANGDPVCNACGLYYKLHNVN RPLTMKKE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	178
15N chemical shifts	64
1H chemical shifts	352

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 68 residues - 7700.792 Da.

1

MET

ALA

HIS

SER

2

GLY

LEU

GLU

VAL

LEU

PHE

GLN

GLY

PRO

ARG

3

ARG

ALA

GLY

THR

CYS

ALA

ASN

CYS

GLN

4

THR

LEU

TRP

ARG

ASN

5

ALA

ASN

GLY

ASP

PRO

VAL

CYS

ASN

ALA

CYS

6

GLY

LEU

TYR

LYS

LEU

HIS

ASN

VAL

ASN

7

ARG

PRO

LEU

THR

MET

LYS

GLU

Entity 2, entity_2 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: GATA2 Zinc Finger 2, [U-99% 13C; U-99% 15N], 10 mg/L

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 Pa; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure calculation

Analysis v2.4.1, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DRX500 500 MHz
Bruker DRX800 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks