BMRB Entry 34037

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34037
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
recombinant mouse Nerve Growth Factor
Deposition date:
2016-08-25
Original release date:
2017-06-30
Authors:
Paoletti, F.; de Chiara, C.; Kelly, G.; Lamba, D.; Cattaneo, A.; Pastore, A.
Citation:

Citation: Paoletti, F.; de Chiara, C.; Kelly, G.; Covaceuszach, S.; Malerba, F.; Yan, R.; Lamba, D.; Cattaneo, A.; Pastore, A.. "Conformational Rigidity within Plasticity Promotes Differential Target Recognition of Nerve Growth Factor."  Front. Mol. Biosci. 3, 83-83 (2016).
PubMed: 28083536

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, 13277.009 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SSTHPVFHMGEFSVCDSVSV WVGDKTTATDIKGKEVTVLA EVNINNSVFRQYFFETKCRA SNPVESGCRGIDSKHWNSYC TTTHTFVKALTTDEKQAAWR FIRIDTACVCVLSRKATR

Data sets:
Data typeCount
13C chemical shifts1008
15N chemical shifts246
1H chemical shifts1514

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_1, 11
2entity_1, 21

Entities:

Entity 1, entity_1, 1 118 residues - 13277.009 Da.

1   SERSERTHRHISPROVALPHEHISMETGLY
2   GLUPHESERVALCYSASPSERVALSERVAL
3   TRPVALGLYASPLYSTHRTHRALATHRASP
4   ILELYSGLYLYSGLUVALTHRVALLEUALA
5   GLUVALASNILEASNASNSERVALPHEARG
6   GLNTYRPHEPHEGLUTHRLYSCYSARGALA
7   SERASNPROVALGLUSERGLYCYSARGGLY
8   ILEASPSERLYSHISTRPASNSERTYRCYS
9   THRTHRTHRHISTHRPHEVALLYSALALEU
10   THRTHRASPGLULYSGLNALAALATRPARG
11   PHEILEARGILEASPTHRALACYSVALCYS
12   VALLEUSERARGLYSALATHRARG

Samples:

sample_1: EDTA 1 mM; Nerve Growth Factor, [U-99% 13C; U-99% 15N], 0.1 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_2
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_2
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_2
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

ARIA v2.3.2, ARIA - refinement, structure calculation

CARA, Keller and Wuthrich - data analysis, peak picking

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks