BMRB Entry 34022

Name: Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein
Published: 2016-12-05

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PDB ID: 5lg9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR34022
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein

Deposition date:

2016-07-06

Original release date:

2016-12-05

Authors:

Benjamin, S.; Matthews, S.

Citation:

Citation: Jia, Y.; Benjamin, S.; Liu, Q.; Xu, Y.; Dogga, S.; Liu, J.; Matthews, S.; Soldati-Favre, D.; Benjamin, S.; Williams, F.; Kerry, L.; Matthews, S.. "Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold." Biochim. Biophys. Acta 1865, 208-219 (2016).
PubMed: 27888074

Assembly members:

Assembly members:
entity_1, polymer, 156 residues, 17607.484 Da.

Natural source:

Natural source: Common Name: Plasmodium falciparum Taxonomy ID: 36329 Superkingdom: Eukaryota Kingdom: not available Genus/species: Plasmodium falciparum

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' Vector: pNIC-ZB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EEKGAYLVFDNASNGTLFIV WKKEKVENALMFIKPTKEVP EFKFVNRNGKNELIRNLQSD KKLFYSGICQFVKEAKDIKG KLTLLQHFDSSFPIKVDLYF LKGSKVMPLNTGEPFVVQDI DAMSVLPKGSSSLKVKTMAK DMFVSRGNTEGASISF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	693
15N chemical shifts	145
1H chemical shifts	1087

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 156 residues - 17607.484 Da.

1

GLU

LYS

GLY

ALA

TYR

LEU

VAL

PHE

ASP

2

ASN

ALA

SER

ASN

GLY

THR

LEU

PHE

ILE

VAL

3

TRP

LYS

GLU

LYS

VAL

GLU

ASN

ALA

LEU

4

MET

PHE

ILE

LYS

PRO

THR

LYS

GLU

VAL

PRO

5

GLU

PHE

LYS

PHE

VAL

ASN

ARG

ASN

GLY

LYS

6

ASN

GLU

LEU

ILE

ARG

ASN

LEU

GLN

SER

ASP

7

LYS

LEU

PHE

TYR

SER

GLY

ILE

CYS

GLN

8

PHE

VAL

LYS

GLU

ALA

LYS

ASP

ILE

LYS

GLY

9

LYS

LEU

THR

LEU

GLN

HIS

PHE

ASP

SER

10

SER

PHE

PRO

ILE

LYS

VAL

ASP

LEU

TYR

PHE

11

LEU

LYS

GLY

SER

LYS

VAL

MET

PRO

LEU

ASN

12

THR

GLY

GLU

PRO

PHE

VAL

GLN

ASP

ILE

13

ASP

ALA

MET

SER

VAL

LEU

PRO

LYS

GLY

SER

14

SER

LEU

LYS

VAL

LYS

THR

MET

ALA

LYS

15

ASP

MET

PHE

VAL

SER

ARG

GLY

ASN

THR

GLU

16

GLY

ALA

SER

ILE

SER

PHE

Samples:

sample_1: DTT 1 mM; HEPES 50 mM; PfIMP2, [U-13C; U-15N], 1.5 mM; sodium azide 0.02%; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure calculation

Analysis, CCPN - peak picking

CNS, Brunger A. T. et.al. - refinement

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker AvanceII 600 MHz
Bruker AvanceII 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks