BMRB Entry 31261

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31261
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
4-module Cysteine Rich Eggshell Membrane Protein (CREMP)
Deposition date:
2025-07-07
Original release date:
2025-12-30
Authors:
Zeinalilathori, S.; Russell, R.; Ross, J.; Modla, S.; Caplan, J.; Polenova, T.; Thorpe, C.
Citation:

Citation: Zeinalilathori, Somayeh; Russell, Ryan; Ross, Jean; Modla, Shannon; Caplan, Jeffrey; Polenova, Tatyana; Thorpe, Colin. "Structure of the disulfide-rich modules of a striking tandem repeat protein, avian cysteine-rich eggshell membrane protein"  Protein Sci. 35, e70431-e70431 (2026).
PubMed: 41432289

Assembly members:

Assembly members:
entity_1, polymer, 121 residues, 13126.137 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGGCNDIYCPKGTTCKMMDG HPQCVQNQPSCSDIQCSKGT TCQMVDGWPRCIQIKAAPSR PSCSDIHCPKGTTCKMIDGH PQCVQNQPTCSDIQCSKGTT CQMVDGWPRCTQLKVAPRRP S

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts109
1H chemical shifts720

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 121 residues - 13126.137 Da.

1   SERGLYGLYCYSASNASPILETYRCYSPRO
2   LYSGLYTHRTHRCYSLYSMETMETASPGLY
3   HISPROGLNCYSVALGLNASNGLNPROSER
4   CYSSERASPILEGLNCYSSERLYSGLYTHR
5   THRCYSGLNMETVALASPGLYTRPPROARG
6   CYSILEGLNILELYSALAALAPROSERARG
7   PROSERCYSSERASPILEHISCYSPROLYS
8   GLYTHRTHRCYSLYSMETILEASPGLYHIS
9   PROGLNCYSVALGLNASNGLNPROTHRCYS
10   SERASPILEGLNCYSSERLYSGLYTHRTHR
11   CYSGLNMETVALASPGLYTRPPROARGCYS
12   THRGLNLEULYSVALALAPROARGARGPRO
13   SER

Samples:

sample_1: 4-mod CREMP, [U-100% 13C; U-100% 15N], 400 uM

sample_2: 4-mod CREMP, [U-100% 13C; U-100% 15N], 400 uM

sample_3: 4-mod CREMP, [U-100% 13C; U-100% 15N], 400 uM

sample_conditions_1: ionic strength: 25 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
HNCAsample_1isotropicsample_conditions_1
HNCACBsample_1isotropicsample_conditions_1
CCCONHsample_1isotropicsample_conditions_1
HNCACOsample_1isotropicsample_conditions_1
HBCBCGCDCEHEsample_1isotropicsample_conditions_1
HBCBCGCDHDsample_1isotropicsample_conditions_1
HCCCONHsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
HBHACONHsample_1isotropicsample_conditions_1
15N-editted NOESYsample_2isotropicsample_conditions_1
13C-editted NOESY (Aliphatic)sample_3isotropicsample_conditions_1
13C-editted NOESY (Aromatic)sample_3isotropicsample_conditions_1

Software:

TopSpin v2.4, Bruker Biospin - collection

NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNmr Analysis v2.4, CCPN - chemical shift assignment

CcpNmr Analysis, CCPN - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

PyMOL, Schrodinger, LLC - refinement

NMR spectrometers:

  • Bruker Bruker NEO spectrometer 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks