BMRB Entry 31238

Name: Solution structure of the Ebola Transcriptional Regulatory Signal - configuration B
Published: 2025-10-07

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PDB ID: 9od4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR31238
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Ebola Transcriptional Regulatory Signal - configuration B

Deposition date:

2025-04-25

Original release date:

2025-10-07

Authors:

Edwards, J.; Kachale, A.; Pham, V.

Citation:

Citation: Edwards, Jerome; Kachale, Ambar; McCleery, Spencer; Law, Gabrielle; Patel, Vivek; D'Souza, Victoria; Pham, Vincent. "The Ebolavirus transcriptional regulatory signal equilibrates between two structural conformations to affect VP30 binding" J. Mol. Biol. ., .-. (2025).
PubMed: 41015113

Assembly members:

Assembly members:
entity_1, polymer, 23 residues, 7294.361 Da.

Natural source:

Natural source: Common Name: Ebola virus Taxonomy ID: 1570291 Superkingdom: Viruses Kingdom: not available Genus/species: Ebola virus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: CUCCUUCUAAUUAUUAAAAG GAG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	157

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 23 residues - 7294.361 Da.

1

C

U

C

U

C

U

A

2

U

A

U

A

G

3

G

A

G

Samples:

sample_1: ribonucleic acid 1.0 mM; Tris HCl 50 mM; NaCl 100 mM; MgCl2 1 mM

sample_2: ribonucleic acid 1.0 mM; Tris HCl 50 mM; NaCl 100 mM; MgCl2 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 100000 Pa; temperature: 311 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRViewJ, NMRFx - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis, structure calculation

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Bruker AVANCE III 800 MHz